Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 49524..50049 Replicon plasmid pJX6-2
Accession NZ_CP064231
Organism Klebsiella pneumoniae strain JX-CR-hvKP-6

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag IS501_RS27420 Protein ID WP_013023785.1
Coordinates 49524..49829 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag IS501_RS27425 Protein ID WP_001568025.1
Coordinates 49831..50049 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS501_RS27390 45219..45845 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
IS501_RS27395 45965..46144 + 180 WP_006788217.1 hypothetical protein -
IS501_RS27400 46600..47394 - 795 WP_004197635.1 site-specific integrase -
IS501_RS27405 47592..48608 - 1017 WP_017899884.1 hypothetical protein -
IS501_RS27410 48619..48933 - 315 WP_053389906.1 hypothetical protein -
IS501_RS27415 48960..49355 - 396 WP_017899885.1 hypothetical protein -
IS501_RS27420 49524..49829 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
IS501_RS27425 49831..50049 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IS501_RS27430 50219..50680 - 462 WP_160880186.1 hypothetical protein -
IS501_RS27435 50637..50867 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
IS501_RS27440 50864..51280 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
IS501_RS27445 51354..52916 + 1563 WP_004206609.1 AAA family ATPase -
IS501_RS27450 52901..53923 + 1023 WP_000361404.1 helicase UvrD -
IS501_RS27455 54179..54876 + 698 Protein_76 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 - 1..105085 105085


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T180611 WP_013023785.1 NZ_CP064231:c49829-49524 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T180611 NZ_CP085619:189157-189264 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT180611 WP_001568025.1 NZ_CP064231:c50049-49831 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT180611 NZ_CP085619:c189108-189043 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References