Detailed information of TA system TA180609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 66009..66679
• Replicon: plasmid pJX6-1
• Accession: NZ_CP064230
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-6

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: IS501_RS26220
• Protein ID: WP_004213072.1
• Coordinates: 66009..66452 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: IS501_RS26225
• Protein ID: WP_004213073.1
• Coordinates: 66449..66679 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS501_RS26185	61420..61695	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
IS501_RS26190	61758..62249	+	492	WP_011251327.1	DM13 domain-containing protein	-
IS501_RS26195	62298..63218	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
IS501_RS26200	63309..63715	+	407	Protein_70	GAF domain-containing protein	-
IS501_RS26205	64230..64865	-	636	Protein_71	response regulator	-
IS501_RS26210	65282..65575	+	294	Protein_72	transposase	-
IS501_RS26215	65579..65995	+	417	Protein_73	restriction endonuclease	-
IS501_RS26220	66009..66452	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
IS501_RS26225	66449..66679	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IS501_RS26230	67287..68420	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
IS501_RS26235	68436..68729	+	294	WP_004213076.1	hypothetical protein	-
IS501_RS26240	68719..68925	-	207	WP_004213077.1	hypothetical protein	-
IS501_RS26245	69277..69567	+	291	WP_004213078.1	hypothetical protein	-
IS501_RS26250	69557..70456	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..228974	228974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T180609 WP_004213072.1 NZ_CP064230:c66452-66009 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T180609 NZ_CP085538:4954-5241 [Aeromonas salmonicida subsp. salmonicida]
ATGACCTTTGAGCTTGAGTTCGACCCACGAGCGTGGAAGGAATGGAAGAAGTTGGGCGATACAGTTCGCCAGCAGTTCAA
AAAGAAGCTGGCAGAGGTGCTGGAGCGACCTAGGGTAGAGGCGAATCGTCTGCATGGGCTGCCGGACTGCTACAAGATTA
AGCTGCGTGGGGCTGGGTACCGGTTGGTGTATCAGGTACAGGATGATCGGGTGCTGGTCTTCGTGGTCGCAGTGGGTAAA
CGAGAGCGGGAGCAGGTGTATCTGGACGCTGGTTATCGGCTGGAATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT180609 WP_004213073.1 NZ_CP064230:c66679-66449 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT180609 NZ_CP085538:4722-4964 [Aeromonas salmonicida subsp. salmonicida]
ATGGGGACGATTAACCTTCGCATCGATGATGAGCTGAAAACCCGCTCGTATGCGGTACTTGAACGACTTGGTGTAACTCC
ATCTGAGTTACTGCGACAAACTCTGGAATATGTGGCCTCTCAGGAACGACTGCCTTTTCGGACGACCGTGGTAACGGAGG
ATGAGGAGGCATTGCTTGTTCTTGTTCGTGAGCGGCTGGCCAGCCCCCAGAAAGGGATCAGGGTGTCGCTGGATGACCTT
TGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620