Detailed information of TA system TA180591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 217..691
• Replicon: plasmid pJX7-5
• Accession: NZ_CP064228
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-7

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: IS505_RS29460
• Protein ID: WP_004197762.1
• Coordinates: 217..483 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: IS505_RS29465
• Protein ID: WP_000879771.1
• Coordinates: 467..691 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS505_RS29460	217..483	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IS505_RS29465	467..691	-	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
IS505_RS29470	1358..1570	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
IS505_RS29475	1577..2086	-	510	WP_032433972.1	MbeB family mobilization protein	-
IS505_RS29480	2061..2825	-	765	Protein_5	relaxase/mobilization nuclease domain-containing protein	-
IS505_RS29485	2764..3108	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
IS505_RS29490	3098..3274	-	177	WP_004197757.1	hypothetical protein	-
IS505_RS29495	4377..4756	+	380	Protein_8	hypothetical protein	-
IS505_RS29500	4782..5327	+	546	WP_023317533.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T180591 WP_004197762.1 NZ_CP064228:c483-217 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T180591 NZ_CP085532:2816531-2816908 [Mycobacterium tuberculosis variant bovis BCG]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT180591 WP_000879771.1 NZ_CP064228:c691-467 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT180591 NZ_CP085532:2816277-2816534 [Mycobacterium tuberculosis variant bovis BCG]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5