Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 43298..43823 Replicon plasmid pJX7-2
Accession NZ_CP064225
Organism Klebsiella pneumoniae strain JX-CR-hvKP-7

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag IS505_RS28275 Protein ID WP_001159871.1
Coordinates 43518..43823 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag IS505_RS28270 Protein ID WP_000813634.1
Coordinates 43298..43516 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS505_RS28235 38504..38920 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
IS505_RS28240 38917..39147 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
IS505_RS28245 39104..39343 + 240 Protein_48 hypothetical protein -
IS505_RS28250 39492..40493 + 1002 WP_000785695.1 DUF4238 domain-containing protein -
IS505_RS28255 40667..41011 - 345 WP_000792769.1 hypothetical protein -
IS505_RS28260 41509..41763 - 255 WP_000678528.1 hypothetical protein -
IS505_RS28265 41813..42739 - 927 WP_001290414.1 hypothetical protein -
IS505_RS28270 43298..43516 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IS505_RS28275 43518..43823 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
IS505_RS28280 43824..44633 + 810 WP_000016963.1 site-specific integrase -
IS505_RS28285 44805..44999 + 195 Protein_56 colicin M immunity protein -
IS505_RS28290 45055..45752 + 698 Protein_57 IS1 family transposase -
IS505_RS28295 45768..46358 - 591 Protein_58 colicin-B -
IS505_RS28300 46538..46964 + 427 Protein_59 tyrosine-type recombinase/integrase -
IS505_RS28305 47156..47431 - 276 WP_000239529.1 hypothetical protein -
IS505_RS28310 47425..48069 - 645 WP_000633913.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / blaLAP-2 / blaKPC-2 / dfrA14 / sul2 - 1..133575 133575
- flank IS/Tn - - 45249..45752 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T180582 WP_001159871.1 NZ_CP064225:43518-43823 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T180582 NZ_CP085532:c2197770-2197351 [Mycobacterium tuberculosis variant bovis BCG]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT180582 WP_000813634.1 NZ_CP064225:43298-43516 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT180582 NZ_CP085532:c2198039-2197779 [Mycobacterium tuberculosis variant bovis BCG]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References