Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 38504..39147 Replicon plasmid pJX7-2
Accession NZ_CP064225
Organism Klebsiella pneumoniae strain JX-CR-hvKP-7

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag IS505_RS28235 Protein ID WP_001044770.1
Coordinates 38504..38920 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag IS505_RS28240 Protein ID WP_001261282.1
Coordinates 38917..39147 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS505_RS28215 34607..34879 - 273 Protein_42 transposase -
IS505_RS28220 34908..35605 - 698 Protein_43 IS1 family transposase -
IS505_RS28225 35861..36883 - 1023 WP_000361404.1 helicase UvrD -
IS505_RS28230 36868..38430 - 1563 WP_004206609.1 AAA family ATPase -
IS505_RS28235 38504..38920 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
IS505_RS28240 38917..39147 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IS505_RS28245 39104..39343 + 240 Protein_48 hypothetical protein -
IS505_RS28250 39492..40493 + 1002 WP_000785695.1 DUF4238 domain-containing protein -
IS505_RS28255 40667..41011 - 345 WP_000792769.1 hypothetical protein -
IS505_RS28260 41509..41763 - 255 WP_000678528.1 hypothetical protein -
IS505_RS28265 41813..42739 - 927 WP_001290414.1 hypothetical protein -
IS505_RS28270 43298..43516 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
IS505_RS28275 43518..43823 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / blaLAP-2 / blaKPC-2 / dfrA14 / sul2 - 1..133575 133575


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T180581 WP_001044770.1 NZ_CP064225:c38920-38504 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T180581 NZ_CP085532:c2188633-2188337 [Mycobacterium tuberculosis variant bovis BCG]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT180581 WP_001261282.1 NZ_CP064225:c39147-38917 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT180581 NZ_CP085532:c2188881-2188630 [Mycobacterium tuberculosis variant bovis BCG]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References