Detailed information of TA system TA180578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 36285..36955
• Replicon: plasmid pJX7-1
• Accession: NZ_CP064224
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-7

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: IS505_RS27035
• Protein ID: WP_004213072.1
• Coordinates: 36512..36955 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: IS505_RS27030
• Protein ID: WP_004213073.1
• Coordinates: 36285..36515 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS505_RS27005	32216..32374	-	159	WP_077250517.1	hypothetical protein	-
IS505_RS27010	32687..33616	+	930	WP_004213558.1	Rpn family recombination-promoting nuclease/putative transposase	-
IS505_RS27015	33761..34540	-	780	WP_004213560.1	site-specific integrase	-
IS505_RS27020	34537..35358	-	822	WP_004213562.1	hypothetical protein	-
IS505_RS27025	35858..36232	-	375	WP_194810313.1	hypothetical protein	-
IS505_RS27030	36285..36515	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IS505_RS27035	36512..36955	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
IS505_RS27040	36969..37385	-	417	Protein_43	restriction endonuclease	-
IS505_RS27045	37389..37682	-	294	Protein_44	transposase	-
IS505_RS27050	38099..38734	+	636	Protein_45	response regulator	-
IS505_RS27055	39249..39655	-	407	Protein_46	GAF domain-containing protein	-
IS505_RS27060	39746..40666	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
IS505_RS27065	40715..41206	-	492	WP_011251327.1	DM13 domain-containing protein	-
IS505_RS27070	41269..41544	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN	1..219796	219796

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T180578 WP_004213072.1 NZ_CP064224:36512-36955 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T180578 NZ_CP085532:1946483-1946731 [Mycobacterium tuberculosis variant bovis BCG]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATACGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT180578 WP_004213073.1 NZ_CP064224:36285-36515 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT180578 NZ_CP085532:1946271-1946483 [Mycobacterium tuberculosis variant bovis BCG]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620