Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5369816..5370441 Replicon chromosome
Accession NZ_CP064223
Organism Klebsiella pneumoniae strain JX-CR-hvKP-7

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IS505_RS26460 Protein ID WP_002882817.1
Coordinates 5369816..5370199 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IS505_RS26465 Protein ID WP_004150355.1
Coordinates 5370199..5370441 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS505_RS26445 5367182..5368084 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IS505_RS26450 5368081..5368716 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IS505_RS26455 5368713..5369642 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IS505_RS26460 5369816..5370199 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IS505_RS26465 5370199..5370441 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IS505_RS26470 5370646..5371563 + 918 WP_002882812.1 alpha/beta hydrolase -
IS505_RS26475 5371577..5372518 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IS505_RS26480 5372563..5373000 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IS505_RS26485 5372997..5373857 - 861 WP_002882807.1 virulence factor BrkB family protein -
IS505_RS26490 5373851..5374450 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T180577 WP_002882817.1 NZ_CP064223:c5370199-5369816 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T180577 NZ_CP085532:c1926042-1925653 [Mycobacterium tuberculosis variant bovis BCG]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT180577 WP_004150355.1 NZ_CP064223:c5370441-5370199 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT180577 NZ_CP085532:c1926266-1926039 [Mycobacterium tuberculosis variant bovis BCG]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References