Detailed information of TA system TA180557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 61260..61819
• Replicon: plasmid pJX8-3
• Accession: NZ_CP064220
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-8

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: IS494_RS29135
• Protein ID: WP_000421257.1
• Coordinates: 61260..61535 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: IS494_RS29140
• Protein ID: WP_001178089.1
• Coordinates: 61535..61819 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS494_RS29100	56439..57830	-	1392	WP_000085084.1	SIR2 family protein	-
IS494_RS29105	57907..58392	-	486	Protein_79	recombinase family protein	-
IS494_RS29110	58456..59160	+	705	WP_001067845.1	IS6-like element IS26 family transposase	-
IS494_RS29115	59247..59594	-	348	WP_000142451.1	hypothetical protein	-
IS494_RS29120	59723..60058	+	336	WP_001080730.1	colicin 1A immunity protein	-
IS494_RS29125	60080..60439	-	360	WP_085452786.1	colicin	-
IS494_RS29130	60513..61210	+	698	Protein_84	IS1 family transposase	-
IS494_RS29135	61260..61535	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IS494_RS29140	61535..61819	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
IS494_RS29145	62463..63467	-	1005	WP_021546935.1	IS110-like element IS4321 family transposase	-
IS494_RS29150	63657..64361	-	705	WP_001067845.1	IS6-like element IS26 family transposase	-
IS494_RS29155	64425..64661	-	237	Protein_89	DUF3330 domain-containing protein	-
IS494_RS29160	64864..65877	-	1014	WP_000845048.1	class 1 integron integrase IntI1	-
IS494_RS29165	66057..66611	+	555	WP_032488579.1	aminoglycoside N-acetyltransferase AAC(6')-Ib3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	cmlA1 / qacE / blaCTX-M-14 / blaTEM-1B	-	1..87179	87179
	-	inside	IScluster/Tn	cmlA1 / qacE / blaCTX-M-14 / blaTEM-1B	-	51158..83815	32657

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T180557 WP_000421257.1 NZ_CP064220:c61535-61260 [Klebsiella pneumoniae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T180557 NZ_CP085532:c698934-698521 [Mycobacterium tuberculosis variant bovis BCG]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT180557 WP_001178089.1 NZ_CP064220:c61819-61535 [Klebsiella pneumoniae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT180557 NZ_CP085532:c699167-698931 [Mycobacterium tuberculosis variant bovis BCG]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: A0A731IHA8