Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 73171..73814 Replicon plasmid pJX8-2
Accession NZ_CP064219
Organism Klebsiella pneumoniae strain JX-CR-hvKP-8

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag IS494_RS28325 Protein ID WP_001044770.1
Coordinates 73398..73814 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag IS494_RS28320 Protein ID WP_001261282.1
Coordinates 73171..73401 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS494_RS28285 68495..68800 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
IS494_RS28290 68802..69020 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
IS494_RS28295 69579..70505 + 927 WP_001290414.1 hypothetical protein -
IS494_RS28300 70555..70809 + 255 WP_000678528.1 hypothetical protein -
IS494_RS28305 71307..71651 + 345 WP_000792769.1 hypothetical protein -
IS494_RS28310 71825..72826 - 1002 WP_000785695.1 DUF4238 domain-containing protein -
IS494_RS28315 72975..73214 - 240 Protein_94 hypothetical protein -
IS494_RS28320 73171..73401 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IS494_RS28325 73398..73814 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
IS494_RS28330 73888..75450 + 1563 WP_004206609.1 AAA family ATPase -
IS494_RS28335 75435..76457 + 1023 WP_000361404.1 helicase UvrD -
IS494_RS28340 76713..77410 + 698 Protein_99 IS1 family transposase -
IS494_RS28345 77439..77711 + 273 Protein_100 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / blaLAP-2 / blaKPC-2 / blaSHV-12 / tet(A) / catA2 / dfrA14 / sul2 - 1..146760 146760


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T180552 WP_001044770.1 NZ_CP064219:73398-73814 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T180552 NZ_CP085532:364781-365083 [Mycobacterium tuberculosis variant bovis BCG]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT180552 WP_001261282.1 NZ_CP064219:73171-73401 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT180552 NZ_CP085532:365131-365352 [Mycobacterium tuberculosis variant bovis BCG]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References