Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 68495..69020 Replicon plasmid pJX8-2
Accession NZ_CP064219
Organism Klebsiella pneumoniae strain JX-CR-hvKP-8

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag IS494_RS28285 Protein ID WP_001159871.1
Coordinates 68495..68800 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag IS494_RS28290 Protein ID WP_000813634.1
Coordinates 68802..69020 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS494_RS28250 64249..64893 + 645 WP_000633913.1 AAA family ATPase -
IS494_RS28255 64887..65162 + 276 WP_000239529.1 hypothetical protein -
IS494_RS28260 65354..65780 - 427 Protein_83 tyrosine-type recombinase/integrase -
IS494_RS28265 65960..66550 + 591 Protein_84 colicin-B -
IS494_RS28270 66566..67263 - 698 Protein_85 IS1 family transposase -
IS494_RS28275 67319..67513 - 195 Protein_86 colicin M immunity protein -
IS494_RS28280 67685..68494 - 810 WP_000016963.1 site-specific integrase -
IS494_RS28285 68495..68800 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
IS494_RS28290 68802..69020 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IS494_RS28295 69579..70505 + 927 WP_001290414.1 hypothetical protein -
IS494_RS28300 70555..70809 + 255 WP_000678528.1 hypothetical protein -
IS494_RS28305 71307..71651 + 345 WP_000792769.1 hypothetical protein -
IS494_RS28310 71825..72826 - 1002 WP_000785695.1 DUF4238 domain-containing protein -
IS494_RS28315 72975..73214 - 240 Protein_94 hypothetical protein -
IS494_RS28320 73171..73401 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
IS494_RS28325 73398..73814 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / blaLAP-2 / blaKPC-2 / blaSHV-12 / tet(A) / catA2 / dfrA14 / sul2 - 1..146760 146760
- flank IS/Tn - - 66566..67069 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T180551 WP_001159871.1 NZ_CP064219:c68800-68495 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T180551 NZ_CP085532:364781-365083 [Mycobacterium tuberculosis variant bovis BCG]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT180551 WP_000813634.1 NZ_CP064219:c69020-68802 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT180551 NZ_CP085532:364557-364784 [Mycobacterium tuberculosis variant bovis BCG]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References