Detailed information of TA system TA180544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 55952..56622
• Replicon: plasmid pJX8-1
• Accession: NZ_CP064218
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-8

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: IS494_RS26960
• Protein ID: WP_004213072.1
• Coordinates: 56179..56622 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: IS494_RS26955
• Protein ID: WP_004213073.1
• Coordinates: 55952..56182 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS494_RS26930	52175..53074	-	900	WP_004225022.1	nucleotide-binding protein	-
IS494_RS26935	53064..53354	-	291	WP_004213078.1	hypothetical protein	-
IS494_RS26940	53706..53912	+	207	WP_004213077.1	hypothetical protein	-
IS494_RS26945	53902..54195	-	294	WP_004213076.1	hypothetical protein	-
IS494_RS26950	54211..55344	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
IS494_RS26955	55952..56182	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IS494_RS26960	56179..56622	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
IS494_RS26965	56636..57052	-	417	Protein_59	restriction endonuclease	-
IS494_RS26970	57056..57349	-	294	Protein_60	transposase	-
IS494_RS26975	57766..58401	+	636	Protein_61	response regulator	-
IS494_RS26980	58916..59322	-	407	Protein_62	GAF domain-containing protein	-
IS494_RS26985	59413..60333	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
IS494_RS26990	60382..60873	-	492	WP_011251327.1	DM13 domain-containing protein	-
IS494_RS26995	60936..61211	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN	1..217869	217869

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T180544 WP_004213072.1 NZ_CP064218:56179-56622 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T180544 NZ_CP085528:75139-75435 [Sinorhizobium meliloti]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTGCCGCCAAGGCCGACCTTCGGAAGATATGGCGCTACAAGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGCAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGACCGGGTTATCTCAAATACCCGGCAGGATCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGGATGGATGCCCAACGGCATTTGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT180544 WP_004213073.1 NZ_CP064218:55952-56182 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT180544 NZ_CP085528:74901-75149 [Sinorhizobium meliloti]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CTTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620