Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5346002..5346627 Replicon chromosome
Accession NZ_CP064217
Organism Klebsiella pneumoniae strain JX-CR-hvKP-8

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IS494_RS26305 Protein ID WP_002882817.1
Coordinates 5346002..5346385 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IS494_RS26310 Protein ID WP_004150355.1
Coordinates 5346385..5346627 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS494_RS26290 5343368..5344270 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IS494_RS26295 5344267..5344902 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IS494_RS26300 5344899..5345828 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IS494_RS26305 5346002..5346385 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IS494_RS26310 5346385..5346627 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IS494_RS26315 5346832..5347749 + 918 WP_002882812.1 alpha/beta hydrolase -
IS494_RS26320 5347763..5348704 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IS494_RS26325 5348749..5349186 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IS494_RS26330 5349183..5350043 - 861 WP_002882807.1 virulence factor BrkB family protein -
IS494_RS26335 5350037..5350636 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T180543 WP_002882817.1 NZ_CP064217:c5346385-5346002 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T180543 NZ_CP085527:1144756-1145031 [Sinorhizobium meliloti]
ATGAAGCTTGAGTGGACGAGTAAGGCAGTCGCCGATCTTGGGCGCCTCTATGATTTCCTGGCACCGGTAAATCGGCAAGC
TGCGGCTCGTACTGTGCAATCATGGACGAGCGCTCCAGCACGCCTGCTCGAACAACCGCGCATCGGCGAACGGCTGGAGG
AATTCGACCCTCGAGAGGTTCGGCGAATTCTCGTTGGCCATTACGAAATACGATATGAGATCCGGCAGTCGACGATCTAC
GTGCTGCGGCTTTGGCATACGCGTGAAGACCGTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT180543 WP_004150355.1 NZ_CP064217:c5346627-5346385 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT180543 NZ_CP085527:1144496-1144756 [Sinorhizobium meliloti]
ATGGAAACGAAGGTGCTGACGGCGCACGTACCACTGCCGCTCGCCGAGAAAGTCGATCAGATCGCGGCCCGGCTTGAGCG
CTCGCGCGGGTGGATCGTCAAGCAGGCGCTGACCGCCTGGGTCGATCAGGAAGAGGAGCGCCGACGCTTGACGCTGGAAG
CGCTTGCCGACGTCGACGCTGGCCGCGTTATCGATCACCAGGCCGTCCAGGCCTGGGCCGACAGTCTTGATAGTGACAAG
CCGCTCTCCTTGCCGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References