Detailed information of TA system TA180525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 63967..64526
• Replicon: plasmid pJX9-3
• Accession: NZ_CP064214
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-9

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: IS495_RS28865
• Protein ID: WP_000421257.1
• Coordinates: 63967..64242 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: IS495_RS28870
• Protein ID: WP_001178089.1
• Coordinates: 64242..64526 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS495_RS28830	59146..60537	-	1392	WP_000085084.1	SIR2 family protein	-
IS495_RS28835	60614..61099	-	486	Protein_82	recombinase family protein	-
IS495_RS28840	61163..61867	+	705	WP_001067845.1	IS6-like element IS26 family transposase	-
IS495_RS28845	61954..62301	-	348	WP_000142451.1	hypothetical protein	-
IS495_RS28850	62430..62765	+	336	WP_001080730.1	colicin 1A immunity protein	-
IS495_RS28855	62787..63146	-	360	WP_085452786.1	colicin	-
IS495_RS28860	63220..63917	+	698	Protein_87	IS1 family transposase	-
IS495_RS28865	63967..64242	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IS495_RS28870	64242..64526	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
IS495_RS28875	65170..66174	-	1005	WP_021546935.1	IS110-like element IS4321 family transposase	-
IS495_RS28880	66364..67068	-	705	WP_001067845.1	IS6-like element IS26 family transposase	-
IS495_RS28885	67132..67368	-	237	Protein_92	DUF3330 domain-containing protein	-
IS495_RS28890	67571..68584	-	1014	WP_000845048.1	class 1 integron integrase IntI1	-
IS495_RS28895	68764..69318	+	555	WP_032488579.1	aminoglycoside N-acetyltransferase AAC(6')-Ib3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	cmlA1 / qacE / blaCTX-M-14 / blaTEM-1B	-	1..90714	90714
	-	inside	IScluster/Tn	cmlA1 / qacE / blaCTX-M-14 / blaTEM-1B	-	53865..87350	33485

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T180525 WP_000421257.1 NZ_CP064214:c64242-63967 [Klebsiella pneumoniae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T180525 NZ_CP085524:914324-914575 [Zhaonella formicivorans]
ATGCAGATTGATGTAATCAGGATTGGTAATTCCAAAGGAGTCCGCATTCCTGCTGCCCTGCTCAAGCAATGTGGAATTGA
CAAGAGGGTTGAGGTTGAAGTCCAGGAGAACTGCATTATTCTTAAGCCGGTTAAGATTCCCAGAGAAGGATGGGCTGCTG
CTTTTAGACGCATGAACAGGAACGGAGACGATGTCCTTTTGCTGCCTGATGAGCTAGATAATGACCTCCTGGAGGAATGG
GATGAATATTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT180525 WP_001178089.1 NZ_CP064214:c64526-64242 [Klebsiella pneumoniae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT180525 NZ_CP085524:914565-914891 [Zhaonella formicivorans]
ATGAATATTAATCAGTATGATGTTTACTGGACCGACTTAAATCCTGCCAAGGGGTCGGAAATAAACAAGGTTCGCCCTTG
TGTAGTGATTTCTCCACCGGAAATGAATGCTTATTTGCGTACTGTTATTGTTGCTCCGGTTACAAGTAAAGGGCGGGAGG
GTTATCCTACACGGGTTTATATGTCTGTAAGTGACGTATACGGCTGGGTTGTGCTTGATCAAATCCGGGCCATTGATAAA
TCGAGGCTGTGCAATAAAATCGGCAGTTTGGACGAAAATGCAGTTTGTGCCATAAAAAGTGTGATTAAGGAAATGTTAGT
AGACTGA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: A0A731IHA8