Detailed information of TA system TA180520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 226769..227439
• Replicon: plasmid pJX9-1
• Accession: NZ_CP064212
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-9

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: IS495_RS27560
• Protein ID: WP_004213072.1
• Coordinates: 226769..227212 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: IS495_RS27565
• Protein ID: WP_004213073.1
• Coordinates: 227209..227439 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS495_RS27525	222180..222455	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
IS495_RS27530	222518..223009	+	492	WP_011251327.1	DM13 domain-containing protein	-
IS495_RS27535	223058..223978	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
IS495_RS27540	224069..224475	+	407	Protein_250	GAF domain-containing protein	-
IS495_RS27545	224990..225625	-	636	Protein_251	response regulator	-
IS495_RS27550	226042..226335	+	294	Protein_252	transposase	-
IS495_RS27555	226339..226755	+	417	Protein_253	restriction endonuclease	-
IS495_RS27560	226769..227212	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
IS495_RS27565	227209..227439	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IS495_RS27570	228047..229180	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
IS495_RS27575	229196..229489	+	294	WP_004213076.1	hypothetical protein	-
IS495_RS27580	229479..229685	-	207	WP_004213077.1	hypothetical protein	-
IS495_RS27585	230037..230327	+	291	WP_004213078.1	hypothetical protein	-
IS495_RS27590	230317..231216	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..277720	277720

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T180520 WP_004213072.1 NZ_CP064212:c227212-226769 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T180520 NZ_CP085506:236487-236837 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT180520 WP_004213073.1 NZ_CP064212:c227439-227209 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT180520 NZ_CP085506:236195-236482 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620