Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 171705..172348 Replicon plasmid pJX9-1
Accession NZ_CP064212
Organism Klebsiella pneumoniae strain JX-CR-hvKP-9

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag IS495_RS27235 Protein ID WP_001044770.1
Coordinates 171932..172348 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag IS495_RS27230 Protein ID WP_001261282.1
Coordinates 171705..171935 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS495_RS27195 167029..167334 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
IS495_RS27200 167336..167554 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
IS495_RS27205 168113..169039 + 927 WP_001290414.1 hypothetical protein -
IS495_RS27210 169089..169343 + 255 WP_000678528.1 hypothetical protein -
IS495_RS27215 169841..170185 + 345 WP_000792769.1 hypothetical protein -
IS495_RS27220 170359..171360 - 1002 WP_000785695.1 DUF4238 domain-containing protein -
IS495_RS27225 171509..171748 - 240 Protein_187 hypothetical protein -
IS495_RS27230 171705..171935 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IS495_RS27235 171932..172348 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
IS495_RS27240 172422..173984 + 1563 WP_004206609.1 AAA family ATPase -
IS495_RS27245 173969..174991 + 1023 WP_000361404.1 helicase UvrD -
IS495_RS27250 175247..175944 + 698 Protein_192 IS1 family transposase -
IS495_RS27255 175973..176245 + 273 Protein_193 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..277720 277720


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T180519 WP_001044770.1 NZ_CP064212:171932-172348 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T180519 NZ_CP085505:c1223733-1222987 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGAATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAAGCGGATAAGGACCGCTTGACAGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTTCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT180519 WP_001261282.1 NZ_CP064212:171705-171935 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT180519 NZ_CP085505:c1222986-1222816 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References