Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 167029..167554 Replicon plasmid pJX9-1
Accession NZ_CP064212
Organism Klebsiella pneumoniae strain JX-CR-hvKP-9

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag IS495_RS27195 Protein ID WP_001159871.1
Coordinates 167029..167334 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag IS495_RS27200 Protein ID WP_000813634.1
Coordinates 167336..167554 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS495_RS27160 162783..163427 + 645 WP_000633913.1 AAA family ATPase -
IS495_RS27165 163421..163696 + 276 WP_000239529.1 hypothetical protein -
IS495_RS27170 163888..164314 - 427 Protein_176 tyrosine-type recombinase/integrase -
IS495_RS27175 164494..165084 + 591 Protein_177 colicin-B -
IS495_RS27180 165100..165797 - 698 Protein_178 IS1 family transposase -
IS495_RS27185 165853..166047 - 195 Protein_179 colicin M immunity protein -
IS495_RS27190 166219..167028 - 810 WP_000016963.1 site-specific integrase -
IS495_RS27195 167029..167334 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
IS495_RS27200 167336..167554 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IS495_RS27205 168113..169039 + 927 WP_001290414.1 hypothetical protein -
IS495_RS27210 169089..169343 + 255 WP_000678528.1 hypothetical protein -
IS495_RS27215 169841..170185 + 345 WP_000792769.1 hypothetical protein -
IS495_RS27220 170359..171360 - 1002 WP_000785695.1 DUF4238 domain-containing protein -
IS495_RS27225 171509..171748 - 240 Protein_187 hypothetical protein -
IS495_RS27230 171705..171935 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
IS495_RS27235 171932..172348 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..277720 277720
- flank IS/Tn - - 165100..165603 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T180518 WP_001159871.1 NZ_CP064212:c167334-167029 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T180518 NZ_CP085505:488723-489073 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT180518 WP_000813634.1 NZ_CP064212:c167554-167336 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT180518 NZ_CP085505:488436-488717 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References