Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5276920..5277545 Replicon chromosome
Accession NZ_CP064211
Organism Klebsiella pneumoniae strain JX-CR-hvKP-9

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IS495_RS25925 Protein ID WP_002882817.1
Coordinates 5276920..5277303 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IS495_RS25930 Protein ID WP_004150355.1
Coordinates 5277303..5277545 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS495_RS25910 5274286..5275188 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IS495_RS25915 5275185..5275820 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IS495_RS25920 5275817..5276746 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IS495_RS25925 5276920..5277303 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IS495_RS25930 5277303..5277545 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IS495_RS25935 5277750..5278667 + 918 WP_002882812.1 alpha/beta hydrolase -
IS495_RS25940 5278681..5279622 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IS495_RS25945 5279667..5280104 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IS495_RS25950 5280101..5280961 - 861 WP_002882807.1 virulence factor BrkB family protein -
IS495_RS25955 5280955..5281554 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T180511 WP_002882817.1 NZ_CP064211:c5277303-5276920 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T180511 NZ_CP085488:c4471401-4471090 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT180511 WP_004150355.1 NZ_CP064211:c5277545-5277303 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT180511 NZ_CP085488:c4471089-4470799 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References