Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4087652..4088271 Replicon chromosome
Accession NZ_CP064211
Organism Klebsiella pneumoniae strain JX-CR-hvKP-9

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag IS495_RS20215 Protein ID WP_002892050.1
Coordinates 4088053..4088271 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag IS495_RS20210 Protein ID WP_002892066.1
Coordinates 4087652..4088026 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS495_RS20200 4082804..4083997 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IS495_RS20205 4084020..4087166 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit -
IS495_RS20210 4087652..4088026 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
IS495_RS20215 4088053..4088271 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
IS495_RS20220 4088430..4088996 + 567 WP_002892030.1 maltose O-acetyltransferase -
IS495_RS20225 4088968..4089108 - 141 WP_004147370.1 hypothetical protein -
IS495_RS20230 4089129..4089599 + 471 WP_002892026.1 YlaC family protein -
IS495_RS20235 4089574..4091025 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
IS495_RS20240 4091126..4091824 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
IS495_RS20245 4091821..4091961 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
IS495_RS20250 4091961..4092224 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T180507 WP_002892050.1 NZ_CP064211:4088053-4088271 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T180507 NZ_CP085488:3395548-3395766 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT180507 WP_002892066.1 NZ_CP064211:4087652-4088026 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT180507 NZ_CP085488:3395148-3395522 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References