Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2733279..2734867 Replicon chromosome
Accession NZ_CP064211
Organism Klebsiella pneumoniae strain JX-CR-hvKP-9

Toxin (Protein)


Gene name hipA Uniprot ID A0A0H3GN97
Locus tag IS495_RS13520 Protein ID WP_004199851.1
Coordinates 2733279..2734601 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag IS495_RS13525 Protein ID WP_002904790.1
Coordinates 2734601..2734867 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS495_RS13505 2728344..2730845 + 2502 WP_004199853.1 fimbrial biogenesis outer membrane usher protein -
IS495_RS13510 2730833..2731837 + 1005 WP_004199852.1 type 1 fimbrial protein -
IS495_RS13515 2731970..2733262 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
IS495_RS13520 2733279..2734601 - 1323 WP_004199851.1 type II toxin-antitoxin system HipA family toxin Toxin
IS495_RS13525 2734601..2734867 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
IS495_RS13530 2735133..2735459 - 327 WP_002904788.1 hydroxyisourate hydrolase -
IS495_RS13535 2735456..2735956 - 501 WP_002904787.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
IS495_RS13540 2735934..2737313 - 1380 WP_004199850.1 purine permease -
IS495_RS13545 2737659..2738813 + 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
IS495_RS13550 2738794..2739717 - 924 WP_004199849.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-402)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49128.44 Da        Isoelectric Point: 7.2487

>T180506 WP_004199851.1 NZ_CP064211:c2734601-2733279 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPLEQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T180506 NZ_CP085488:2637778-2637885 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT180506 WP_002904790.1 NZ_CP064211:c2734867-2734601 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT180506 NZ_CP085488:c2637728-2637665 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GN97

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References