Detailed information of TA system TA180493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 103403..104073
• Replicon: plasmid pJX11-1
• Accession: NZ_CP064209
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-11

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: IS500_RS26080
• Protein ID: WP_004213072.1
• Coordinates: 103403..103846 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: IS500_RS26085
• Protein ID: WP_004213073.1
• Coordinates: 103843..104073 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS500_RS26045	98812..99087	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
IS500_RS26050	99150..99641	+	492	WP_011251327.1	DM13 domain-containing protein	-
IS500_RS26055	99690..100610	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
IS500_RS26060	100701..101107	+	407	Protein_116	GAF domain-containing protein	-
IS500_RS26065	101622..102257	-	636	Protein_117	response regulator	-
IS500_RS26070	102676..102969	+	294	Protein_118	transposase	-
IS500_RS26075	102973..103389	+	417	Protein_119	restriction endonuclease	-
IS500_RS26080	103403..103846	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
IS500_RS26085	103843..104073	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IS500_RS26090	104681..105814	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
IS500_RS26095	105830..106123	+	294	WP_004213076.1	hypothetical protein	-
IS500_RS26100	106113..106319	-	207	WP_004213077.1	hypothetical protein	-
IS500_RS26105	106671..106961	+	291	WP_004213078.1	hypothetical protein	-
IS500_RS26110	106951..107850	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..228028	228028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T180493 WP_004213072.1 NZ_CP064209:c103846-103403 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T180493 NZ_CP085483:314-580 [Proteus sp. NMG38-2]
TTGGTCTGGACGATTAATTATTCTGAACGGGCGTTAAAATATCTACGAAAAATGGATAAACAAAATGCCCGTCGTATTGT
CGATTTCATGGATTTACGCATTGCAGCTTTAGATGACCCCCGACAGTCAGGAAAACCACTCAAAGGTGAACTGGGTGAGT
TCTGGCGTTATAGGGTTGGTGACTATCGGATACTGTGCGAAATAAGGGATAATGAGCTTATTATTCTTGCTACAACTATA
GGACATAGAAAATCAGTCTATGATTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT180493 WP_004213073.1 NZ_CP064209:c104073-103843 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT180493 NZ_CP085483:106-330 [Proteus sp. NMG38-2]
ATGTTAGCTATTCGTTTATCTGATGACATTGAAACACGTCTGGAGTCACTCGCCAAACAGACAGGAAGAACCAAAACATT
CTATGCGAGAGAAGCTATCCTCACTCATTTGGAAGATTTGGAAGACTACTACCTGTCCGCTGAAATCGTTGCAAAAATTC
GTCGCGGTGAAGAACAAGTTCATTCCGCTGAAGATGTGAGGAAAGCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620