Detailed information of TA system TA180492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 50791..51701
• Replicon: plasmid pJX11-1
• Accession: NZ_CP064209
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-11

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: IS500_RS25785
• Protein ID: WP_004026354.1
• Coordinates: 50791..51261 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: IS500_RS25790
• Protein ID: WP_004026357.1
• Coordinates: 51258..51701 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS500_RS25765	46203..46601	-	399	WP_004212796.1	H-NS histone family protein	-
IS500_RS25770	46938..47432	-	495	WP_004212794.1	thermonuclease family protein	-
IS500_RS25775	47610..48559	+	950	Protein_59	DUF1173 family protein	-
IS500_RS25780	49945..50642	-	698	Protein_60	IS1 family transposase	-
IS500_RS25785	50791..51261	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
IS500_RS25790	51258..51701	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
IS500_RS25795	51802..52305	-	504	Protein_63	DUF4113 domain-containing protein	-
IS500_RS25800	52403..52624	-	222	WP_004213643.1	DUF2188 domain-containing protein	-
IS500_RS25805	52769..52924	-	156	WP_004213642.1	hypothetical protein	-
IS500_RS25810	52999..55125	-	2127	WP_004213640.1	TonB-dependent receptor family protein	-
IS500_RS25815	55212..56165	-	954	Protein_67	fec operon regulator FecR	-
IS500_RS25820	56162..56683	-	522	WP_004213635.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	49945..50448	503
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..228028	228028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T180492 WP_004026354.1 NZ_CP064209:c51261-50791 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T180492 NZ_CP085482:c262387-262076 [Proteus sp. NMG38-2]
ATGCACGTAATATCAAGAGAACCTTTTGACCAGGCATCCAAGCGATTTCCAAACAGTGCTCAGGCGCTATCTGACATATA
TCGAACACTCAAGCGCGACAGTTATCAAACGCCTGATGAGCTTAAAAAGGTTTTCCCCAGCCTTGATCGGATGAAGTATA
GAGAGAAATGGTGGGTCATCGACATCAGTGGCAATTCATTAAGAATGATGTTTTTTGCCGACTTTGACCGGGGGAAAATA
TTCGTGAAACATATAACAACCCACGCTGAATACGATAGGTTGACTGATCACTACCGGAGAACAAAAGCATGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT180492 WP_004026357.1 NZ_CP064209:c51701-51258 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT180492 NZ_CP085482:c262079-261663 [Proteus sp. NMG38-2]
ATGATGTTTCAAGATGCAGTAAACGCAGCACAAAACCTGATAAGCATTGTGCCGTTATTGGGAAACAGCCATTCTCGCGA
AGACTATGAAAAAGCCGTTCAACTGGTTGAGCACCTTGTCGAAAATGACCCAGACAACCCGCTTATCGACTTGATTTGCG
CGAAGATTGATGCGTATGAAAAAACAGCGCCCGAATTTGCTGAATTTAACGAGCGCTTAGCAAAAAGCAATGGCGGTGTA
GCAGCATTGAGAACGCTGATGGATCAATACCACCTTAATACGACAGACTTTCAAGATGAGCTCGGTTCGCGCTCATACGT
TAGCCGCATCCTCAACGGTGAACGAGGATTAACACTGGAGCACATTAAAAAACTGTCTGCGCGTTTTAATATCCCTGCCT
CCATTTTCATAGACTAA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9