Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5265395..5266020 Replicon chromosome
Accession NZ_CP064208
Organism Klebsiella pneumoniae strain JX-CR-hvKP-11

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag IS500_RS25115 Protein ID WP_012737091.1
Coordinates 5265395..5265778 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IS500_RS25120 Protein ID WP_004150355.1
Coordinates 5265778..5266020 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS500_RS25100 5262761..5263663 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IS500_RS25105 5263660..5264295 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IS500_RS25110 5264292..5265221 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IS500_RS25115 5265395..5265778 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
IS500_RS25120 5265778..5266020 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IS500_RS25125 5266225..5267142 + 918 WP_012737090.1 alpha/beta hydrolase -
IS500_RS25130 5267156..5268097 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IS500_RS25135 5268142..5268579 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IS500_RS25140 5268576..5269436 - 861 WP_004146232.1 virulence factor BrkB family protein -
IS500_RS25145 5269430..5270029 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T180490 WP_012737091.1 NZ_CP064208:c5265778-5265395 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T180490 NZ_CP085481:c803671-803468 [Proteus sp. NMG38-2]
ATGACTAAGACAGACTATCTAATGCGTTTAAGAAAATGTACCTCAATCGAAACATTAGAGCGAGTAATTGAAAAAAACAA
ATACGAACTCTCTGATGATGAGCTGGAGCTATTTTATTCAGCGGCTGATCATCGTTTAGCTGAGCTCACTATGAATAAAC
TCTATGACAAAATTCCAGCATCTGTTTGGAAATTTGTAAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT180490 WP_004150355.1 NZ_CP064208:c5266020-5265778 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT180490 NZ_CP085481:c804080-803718 [Proteus sp. NMG38-2]
ATGGACGAATATTCACCAGAAAAAGTAGATTTAGCTGAATTGTCGTTTTTATGTGAAGAGCTATTGCAGCAAGCTTTAGG
CTCATTAGATAAAGGCAACACCGTTTGGCACAATGATCTGAGTAGCACAAAAAGCGTTGACCTTAATGTCTTAATTGAAC
ACATTATGGACTTTAGTTGGAAATTTAAGATTAAATACCCTGATAAACAAGTAATTAATACTCTTATAGAAGAGTACCTT
GAAGAAACCTATCGTTTATTTGGTAATACATCAATTTCTTACTCAGATATCAATAATTGGAAAGAGTTAAATCATTCACT
TTTAACCCTTATAGCCAAAAACCCAAAAAGTTATATTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References