Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4626942..4627752 Replicon chromosome
Accession NZ_CP064208
Organism Klebsiella pneumoniae strain JX-CR-hvKP-11

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag IS500_RS22140 Protein ID WP_014908042.1
Coordinates 4626942..4627475 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag IS500_RS22145 Protein ID WP_002887278.1
Coordinates 4627486..4627752 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS500_RS22135 4625773..4626894 + 1122 WP_012737335.1 cupin domain-containing protein -
IS500_RS22140 4626942..4627475 - 534 WP_014908042.1 GNAT family N-acetyltransferase Toxin
IS500_RS22145 4627486..4627752 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
IS500_RS22150 4627855..4629288 - 1434 WP_014908043.1 Cu(+)/Ag(+) sensor histidine kinase -
IS500_RS22155 4629278..4629961 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
IS500_RS22160 4630133..4631518 + 1386 WP_012737332.1 efflux transporter outer membrane subunit -
IS500_RS22165 4631536..4631880 + 345 WP_012737331.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T180488 WP_014908042.1 NZ_CP064208:c4627475-4626942 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T180488 NZ_CP085479:c88378-87896 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGATGTCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGAAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT180488 WP_002887278.1 NZ_CP064208:c4627752-4627486 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT180488 NZ_CP085479:c88647-88369 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACACCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References