Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5392503..5393128 Replicon chromosome
Accession NZ_CP064205
Organism Klebsiella pneumoniae strain JX-CR-hvKP-12

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag IS504_RS25925 Protein ID WP_012737091.1
Coordinates 5392503..5392886 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IS504_RS25930 Protein ID WP_004150355.1
Coordinates 5392886..5393128 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS504_RS25910 5389869..5390771 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IS504_RS25915 5390768..5391403 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IS504_RS25920 5391400..5392329 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IS504_RS25925 5392503..5392886 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
IS504_RS25930 5392886..5393128 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IS504_RS25935 5393333..5394250 + 918 WP_012737090.1 alpha/beta hydrolase -
IS504_RS25940 5394264..5395205 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IS504_RS25945 5395250..5395687 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IS504_RS25950 5395684..5396544 - 861 WP_004146232.1 virulence factor BrkB family protein -
IS504_RS25955 5396538..5397137 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T180471 WP_012737091.1 NZ_CP064205:c5392886-5392503 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T180471 NZ_CP085468:c2230632-2230378 [Aeromonas caviae]
ATGAGATTAATTTTTTCCGATGATGCATGGGAAGATTATCTGTTCTGGCAAAAACAAGACCGCCGCATGGTTGATCGAAT
TAACAAGCTAATTAAAGAAACCACCCGAGAGCCTTTCTCTGGAGTGGGCAAGCCAGAGCCATTAAAACATGCTTTGGCCG
GTTATTGGTCTCGTCGAATTACGGATGAGCACCGAATGGTTTACAAAGTGGCGGATGATGCCCTTTGGATCGTTCAACTC
AAGTACCACTACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT180471 WP_004150355.1 NZ_CP064205:c5393128-5392886 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT180471 NZ_CP085468:c2230880-2230629 [Aeromonas caviae]
ATGGACGCTATCACTTACACACACGCCCGCGCCAACCTGGCTGGAACCATGGATCGTGTTTGCAACGATCATGAGCCGGT
GATCATCACACGTAACGGCGACCAATCGGTTGTGATTCTCTCGCTGGAAGATTTCCAGGCGCTGGAAGAAACTGCGTATC
TGCTGCGCAGCCCCGCCAACGCCAAGCGGCTTTTCAAGTCGATCGAGCAACTTGAAGCGGGCCGAGGACAGGCGCGGGAG
TTGGCCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References