Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4164191..4164810 Replicon chromosome
Accession NZ_CP064205
Organism Klebsiella pneumoniae strain JX-CR-hvKP-12

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag IS504_RS20120 Protein ID WP_002892050.1
Coordinates 4164592..4164810 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag IS504_RS20115 Protein ID WP_002892066.1
Coordinates 4164191..4164565 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS504_RS20105 4159343..4160536 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IS504_RS20110 4160559..4163705 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
IS504_RS20115 4164191..4164565 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
IS504_RS20120 4164592..4164810 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
IS504_RS20125 4164969..4165535 + 567 WP_002892030.1 maltose O-acetyltransferase -
IS504_RS20130 4165507..4165647 - 141 WP_004147370.1 hypothetical protein -
IS504_RS20135 4165668..4166138 + 471 WP_002892026.1 YlaC family protein -
IS504_RS20140 4166113..4167564 - 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
IS504_RS20145 4167665..4168363 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
IS504_RS20150 4168360..4168500 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
IS504_RS20155 4168500..4168763 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T180468 WP_002892050.1 NZ_CP064205:4164592-4164810 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T180468 NZ_CP085431:200907-201257 [Priestia megaterium]
TTGGTAGTCAAACGTGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATTCAAAATGATATAGGCAATCGTTTTAGCCCAACTGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAGTTACCTACGCATGTTGAAATTGATGCAAAGCGCTATGGATTTGAGCGAGATTCAGTTATTTTACTAGAACAAATT
CGTACTATTGATAAACAGAGGCTAACAGACAAGATTACCCATTTAGATGATGAAATGATGGACAGAGTGGATGAAGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT180468 WP_002892066.1 NZ_CP064205:4164191-4164565 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT180468 NZ_CP085431:200621-200902 [Priestia megaterium]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTAGTAAGATTACCGAAAAATTTAGTATCGGAGCTCGATCTTATGGTTAA
ACAAGAAAATGGCAACCGAAGTGATTTTATTCACCAAGCAACAAAATTGTACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAATCAATGAGACGTGGATATATGGAGATGGCAAAGATAAACTTAAATATTGCTTCCGAGGCTTTTCTAGCAGAGTAT
GAAGCTGATCATACTGTGGATCGTTTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References