Detailed information of TA system TA180450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 945187..945754
• Replicon: chromosome
• Accession: NZ_CP064192
• Organism: Saccharothrix sp. 6-C

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IOD16_RS04450
• Protein ID: WP_123743528.1
• Coordinates: 945404..945754 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: IOD16_RS04445
• Protein ID: WP_123743529.1
• Coordinates: 945187..945417 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD16_RS04415	940396..940626	-	231	WP_123743534.1	hypothetical protein	-
IOD16_RS04425	940956..941801	+	846	WP_123743533.1	DUF2236 domain-containing protein	-
IOD16_RS04435	942640..943701	+	1062	WP_201438979.1	sigma-70 family RNA polymerase sigma factor	-
IOD16_RS04440	943758..945164	+	1407	WP_201441195.1	MFS transporter	-
IOD16_RS04445	945187..945417	+	231	WP_123743529.1	ribbon-helix-helix domain-containing protein	Antitoxin
IOD16_RS04450	945404..945754	+	351	WP_123743528.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IOD16_RS04455	945806..946000	-	195	WP_123743527.1	ferredoxin	-
IOD16_RS04460	945997..947196	-	1200	WP_201438980.1	cytochrome P450	-
IOD16_RS04465	947383..948255	+	873	WP_201441196.1	ABC transporter ATP-binding protein	-
IOD16_RS04470	948252..949103	+	852	WP_123743525.1	ABC transporter permease	-
IOD16_RS04475	949100..949339	+	240	WP_123743524.1	helix-turn-helix transcriptional regulator	-
IOD16_RS04480	949378..950532	-	1155	WP_201438981.1	glycosyl transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12238.12 Da
• Isoelectric Point: 10.7059

>T180450 WP_123743528.1 NZ_CP064192:945404-945754 [Saccharothrix sp. 6-C]
MRRGDIYWVDLEPAQGAEANKIRPAVVVSNDAANRAAGRGGGRGVVTVVPITSNTAKVYPFQVLLPAKDCGLGADSKAQA
EQVRTVTAARLRARIGGLPPAVLKQLDEALRVHLSL

Nucleotide

• Length: 351 bp

>T180450 NZ_CP085393:c61847-61443 [Cytobacillus oceanisediminis]
ATGAAAAGTGATGTAATTTTAAATAAAATAAATATAATTGAACGCTGTATTAAACGAATTCACGAGGAATACGACCAGAA
TCCTAAAAATCTAGAGAATTATACAAAGCAAGATTCAATTATTTTAAATCTGCAGCGGGCTTGTGAGGCAAGTATTGACT
TGGCCATGCACATAGTAGCCGTAAAAAAACTGGGACTCCCACAACAAAGCCGGGATGCATTTACCTTATTGGAAGCTGAG
GGTATTATTCCCTCCTCTCTTTCAGATAAGATGAAAGCTATGGTTGGATTTCGTAATATTGCAGTTCATGATTACCAAGA
GATTAATTTAGTGATCCTTCAAAAGATACTTGATAATCATTTAGTGGATTTCTTTGAATATACAAAAACCATTTTAATGC
ACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8490.35 Da
• Isoelectric Point: 4.2080

>AT180450 WP_123743529.1 NZ_CP064192:945187-945417 [Saccharothrix sp. 6-C]
MKISVSLPEEDVEFVDRYLRQHRSASRSSVIHQAITLLREAGLENAYASAWEEWEAGGDADMWDVTAPDGVLDAAR

Nucleotide

• Length: 231 bp

>AT180450 NZ_CP085393:c62244-61837 [Cytobacillus oceanisediminis]
TTGAATCCACACCTTGAAGAAACCATTATAGATGTACTTCGTGAAAAATTATCCCCCGATTGGATAGTTATGTTTGGTTC
AACCGTTAGTGGAAATACCCATAAAGAAAGTGACATTGATATTGCATATTTGAGTGACACAATTAAAGCAGATAAATATC
AAATATTTATGATTGCTCAGGAATTAGCAGCAAAACTCAACAAAGATGTGGACCTCATTGATTTAAATCAGGCCAGTACA
GTATTTCAAGCCCAAATAGTTCAATCCGGAACAACTATTTATTGTACCAATGAGCGAAAAAAAGCTGAATTTGAAGTTAA
AGTATTAAAAATGTATACGAAGCTAAATGAGGAGCGATCAGAGATATTAAAAAAAATTGACGAAAGCGGGACAATATATG
AAAAGTGA