Detailed information of TA system TA180447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 339722..340266
• Replicon: plasmid unnamed4
• Accession: NZ_CP064191
• Organism: Rhizobium sp. 007

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ISN39_RS35555
• Protein ID: WP_194732557.1
• Coordinates: 339943..340266 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ISN39_RS35550
• Protein ID: WP_194732556.1
• Coordinates: 339722..339946 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISN39_RS35530	335739..336479	-	741	WP_194732555.1	response regulator	-
ISN39_RS35535	336483..337215	-	733	Protein_318	NodA family N-acyltransferase	-
ISN39_RS35540	337446..338123	-	678	Protein_319	IS630 family transposase	-
ISN39_RS35545	338194..339175	+	982	Protein_320	IS5 family transposase	-
ISN39_RS35550	339722..339946	+	225	WP_194732556.1	antitoxin MazE family protein	Antitoxin
ISN39_RS35555	339943..340266	+	324	WP_194732557.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ISN39_RS35560	340456..341829	+	1374	Protein_323	ISKra4 family transposase	-
ISN39_RS35565	342126..342872	-	747	WP_194732161.1	4'-phosphopantetheinyl transferase superfamily protein	-
ISN39_RS35570	342935..344290	-	1356	WP_194732558.1	MATE family efflux transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..441467	441467
	-	flank	IS/Tn	-	-	338471..339175	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (7-71)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11445.37 Da
• Isoelectric Point: 10.2912

>T180447 WP_194732557.1 NZ_CP064191:339943-340266 [Rhizobium sp. 007]
VRRGDLVTVAMPGDFGKPRPALIIQADQFEDTGTVTVLLVSGTLVDAPLIRPTVRPTPENGLRKPSQVMIDTAMSVKRDK
LGPSFGRLDDGTMLSITRSLAVFLGLA

Nucleotide

• Length: 324 bp

>T180447 NZ_CP085390:257627-257977 [Cytobacillus firmus]
TTGATTGTCAAACGTGGTGACGTTTATTTTGCAGACCTATCCCCAGTTGTTGGTTCAGAACAAGGCGGCGTCCGTCCAGT
GCTTGTCATCCAAAACGACATCGGGAATCGGTTTAGTCCCACAGTAATTGTTGCAGCAATCACAGCTCAGATTCAAAAAG
CAAAGCTGCCTACTCACGTTGAAATTGATGCGAAGCGTTATGGTTTTGAAAGGGATTCGGTCATCTTATTAGAACAGATT
CGTACAATTGATAAACAACGCTTAACCGATAAAATAACCCATCTCGATGACGAAATGATGGAAAAAGTGGATGAAGCCGT
GCAAATCAGCTTAGGCCTCATCGAATTTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8121.09 Da
• Isoelectric Point: 4.5161

>AT180447 WP_194732556.1 NZ_CP064191:339722-339946 [Rhizobium sp. 007]
MPTPVTQRVQKRRDALRAAGLRPVQIWVPDTRLPGFADECRRQSRAVAAADAADRDLDAFMDAALADLDDEADA

Nucleotide

• Length: 225 bp

>AT180447 NZ_CP085390:257341-257622 [Cytobacillus firmus]
GTGTCTGAGTCCAGCACAACAACAGAGATAATGGTGAAATTACCGCAGCATCTTTTAACCGAGTTAGACGGATTTGCTAA
ACAGGAAAACGTTAACCGGAGCGAATTCATTTATCAAGCAACCAAAATGTATTTGCGTGAACGCAAAAAGAGACAAATTC
GCGAGTCCATGAGACGTGGTTACATGGAGATGGCGAAAATAAATTTGACGATTGCATCTGAAGCATTTCAAGCGGAATAC
GAGGCAGAACACACAGTTGAACGTCTAGTTAGCGGAGGATAA