Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 306712..307312 Replicon plasmid unnamed4
Accession NZ_CP064191
Organism Rhizobium sp. 007

Toxin (Protein)


Gene name PumA Uniprot ID -
Locus tag ISN39_RS35400 Protein ID WP_194732538.1
Coordinates 306712..307002 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag ISN39_RS35405 Protein ID WP_194732539.1
Coordinates 307004..307312 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISN39_RS35375 301994..302338 + 345 WP_194732536.1 IS66 family insertion sequence element accessory protein TnpB -
ISN39_RS35380 302413..304031 + 1619 Protein_287 IS66 family transposase -
ISN39_RS35385 304347..304528 - 182 Protein_288 transposase -
ISN39_RS35390 305482..305690 + 209 Protein_289 IS3 family transposase -
ISN39_RS35395 306108..306350 - 243 WP_194732537.1 hypothetical protein -
ISN39_RS35400 306712..307002 + 291 WP_194732538.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ISN39_RS35405 307004..307312 + 309 WP_194732539.1 putative addiction module antidote protein Antitoxin
ISN39_RS35410 307553..308575 - 1023 Protein_293 ISNCY family transposase -
ISN39_RS35415 308551..309221 - 671 Protein_294 transposase -
ISN39_RS35420 309114..309362 + 249 WP_194732657.1 hypothetical protein -
ISN39_RS35425 309381..310145 + 765 Protein_296 IS3 family transposase -
ISN39_RS35430 310508..311452 - 945 WP_194732644.1 omptin family outer membrane protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB 1..441467 441467


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(4-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(50-90)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 10947.71 Da        Isoelectric Point: 10.2193

>T180446 WP_194732538.1 NZ_CP064191:306712-307002 [Rhizobium sp. 007]
MIEVRLTVVFSRWLEDLKDRRARDRVLARLRRFELENFGDAKPVGSGVSEARIDYGPGYRLYFVRQADVVVVMLCGGDKA
SQKRDIATAKAMAKEL

Download         Length: 291 bp

>T180446 NZ_CP085389:554833-555183 [Bacillus altitudinis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACGATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 10860.34 Da        Isoelectric Point: 7.0095

>AT180446 WP_194732539.1 NZ_CP064191:307004-307312 [Rhizobium sp. 007]
MALETTRFDIQNYIKSHEDQTGYLDAVLEDGDPALIAAAIGDIARARGASQFAKDSGLSRETIYKVFRPGGNPTLDTIAK
AVKVLGLRLSLVPASNSTKGPI

Download         Length: 309 bp

>AT180446 NZ_CP085389:554547-554828 [Bacillus altitudinis]
TTGTCTGAATCCAGCGCAACAAATGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References