Detailed information of TA system TA180445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 279633..280285
• Replicon: plasmid unnamed4
• Accession: NZ_CP064191
• Organism: Rhizobium sp. 007

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ISN39_RS35260
• Protein ID: WP_194732519.1
• Coordinates: 279884..280285 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: ISN39_RS35255
• Protein ID: WP_194732518.1
• Coordinates: 279633..279887 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISN39_RS35235	274797..275630	+	834	WP_194732514.1	carbohydrate ABC transporter permease	-
ISN39_RS35240	275671..276927	+	1257	WP_194732515.1	ABC transporter substrate-binding protein	-
ISN39_RS35245	276990..277799	+	810	WP_194732516.1	phosphodiesterase	-
ISN39_RS35250	277796..278482	+	687	WP_194732517.1	HAD family hydrolase	-
ISN39_RS35255	279633..279887	+	255	WP_194732518.1	antitoxin	Antitoxin
ISN39_RS35260	279884..280285	+	402	WP_194732519.1	type II toxin-antitoxin system VapC family toxin	Toxin
ISN39_RS35265	280361..280529	-	169	Protein_264	IS6 family transposase	-
ISN39_RS35270	280853..281713	+	861	WP_194732520.1	sulfotransferase domain-containing protein	-
ISN39_RS35275	282026..283504	-	1479	WP_194732521.1	sulfatase-like hydrolase/transferase	-
ISN39_RS35280	283798..284646	-	849	WP_194732522.1	MurR/RpiR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..441467	441467

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (15-52)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14252.34 Da
• Isoelectric Point: 5.6556

>T180445 WP_194732519.1 NZ_CP064191:279884-280285 [Rhizobium sp. 007]
MTRYLLDTNIISDLVRNPGGLATKHLQRAGDENVCTSIIVAAELRYGCAKKGSAKLLERVEEVLSAIPVLALEGPADIVY
GSIRATLEREGRTVGHNGLLIAAHASSLGTTLVTANVGEFSRIPDLTIENWIE

Nucleotide

• Length: 402 bp

>T180445 NZ_CP085388:c1220961-1220215 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGGACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9604.87 Da
• Isoelectric Point: 4.5317

>AT180445 WP_194732518.1 NZ_CP064191:279633-279887 [Rhizobium sp. 007]
MVHAHQPDSKEVRLFRNNRSQAIRIPAEFELPGERVVISREGDRLVIEPVRTSTGLLNLLASWSPLDEEFPDIEDAPVKP
EEIF

Nucleotide

• Length: 255 bp

>AT180445 NZ_CP085388:c1220214-1220044 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA