Detailed information of TA system TA180444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 246801..247438
• Replicon: plasmid unnamed2
• Accession: NZ_CP064189
• Organism: Rhizobium sp. 007

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ISN39_RS32675
• Protein ID: WP_194732120.1
• Coordinates: 247037..247438 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: ISN39_RS32670
• Protein ID: WP_194732119.1
• Coordinates: 246801..247037 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISN39_RS32650	242765..243082	-	318	WP_145611386.1	hypothetical protein	-
ISN39_RS32655	243729..244334	-	606	WP_022719486.1	4'-phosphopantetheinyl transferase superfamily protein	-
ISN39_RS32660	245416..246105	-	690	Protein_171	IS6 family transposase	-
ISN39_RS32665	246155..246559	+	405	Protein_172	IS5/IS1182 family transposase	-
ISN39_RS32670	246801..247037	+	237	WP_194732119.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ISN39_RS32675	247037..247438	+	402	WP_194732120.1	type II toxin-antitoxin system VapC family toxin	Toxin
ISN39_RS32680	247515..248495	+	981	WP_194732121.1	IS5 family transposase	-
ISN39_RS32685	248652..249884	-	1233	WP_194732122.1	DUF3422 family protein	-
ISN39_RS32690	249900..251255	-	1356	WP_194732223.1	Glu/Leu/Phe/Val dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..461509	461509

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-117)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14864.11 Da
• Isoelectric Point: 5.1660

>T180444 WP_194732120.1 NZ_CP064189:247037-247438 [Rhizobium sp. 007]
MPRYMLDTNMCIYLMKNQPEQVARRFASCYVGDVVMSAITFAELEYGVSVSDDPARELDNLAALAELITVEPFGIAAARA
YGPVRLATRDRKKDHLDKLIASHAIALNTVLVTNNTRDFLAYPGLRLENWLDA

Nucleotide

• Length: 402 bp

>T180444 NZ_CP085388:479167-479517 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8975.10 Da
• Isoelectric Point: 6.2271

>AT180444 WP_194732119.1 NZ_CP064189:246801-247037 [Rhizobium sp. 007]
MHTTKAFKNGNSQAVRIPAELAFDRTDVELEIERVGDELRIRPVHRSLAGVLDVFARFSPDFMRDGREPHEQGERDKI

Nucleotide

• Length: 237 bp

>AT180444 NZ_CP085388:478880-479161 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA