Detailed information of TA system TA180441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4057905..4058904
• Replicon: chromosome
• Accession: NZ_CP064187
• Organism: Rhizobium sp. 007

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: ISN39_RS19715
• Protein ID: WP_194730262.1
• Coordinates: 4058347..4058904 (+)
• Length: 186 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: ISN39_RS19710
• Protein ID: WP_183906422.1
• Coordinates: 4057905..4058351 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISN39_RS19685	4054030..4055730	-	1701	WP_022713201.1	30S ribosomal protein S1	-
ISN39_RS19690	4055907..4056551	-	645	WP_074066193.1	(d)CMP kinase	-
ISN39_RS19695	4056548..4056769	-	222	Protein_3881	FMN-binding negative transcriptional regulator	-
ISN39_RS19700	4057053..4057436	+	384	WP_022713204.1	TIGR02300 family protein	-
ISN39_RS19710	4057905..4058351	+	447	WP_183906422.1	helix-turn-helix domain-containing protein	Antitoxin
ISN39_RS19715	4058347..4058904	+	558	WP_194730262.1	PIN domain-containing protein	Toxin
ISN39_RS19720	4059184..4060308	+	1125	WP_194728596.1	DNA replication/repair protein RecF	-
ISN39_RS19725	4060305..4061129	+	825	WP_074066197.1	molybdopterin-synthase adenylyltransferase MoeB	-
ISN39_RS19730	4061126..4061599	+	474	WP_194728597.1	GNAT family N-acetyltransferase	-
ISN39_RS19735	4061596..4062597	-	1002	WP_074066199.1	D-glycerate dehydrogenase	-
ISN39_RS19740	4062784..4063323	+	540	WP_194728598.1	SH3 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-114)

Antitoxin

• SinI-like, DNA-binding domain (78-122)

Sequences

Toxin

Protein

• Length: 186 a.a.
• Molecular weight: 20500.18 Da
• Isoelectric Point: 5.2482

>T180441 WP_194730262.1 NZ_CP064187:4058347-4058904 [Rhizobium sp. 007]
IKKSSYTVILDACVLYPAPLRDFLMELAAAGQFRAKWSDEIHDEWTRNVLKNRDDLTIEQLGRTKDLMNAAVPDSVVDGY
SDLVPALALPDPDDRHVLAAAIVSASDAIVTFNLSDFPRKTVSQYDIEVLHPDDFIHHQFGLNNAGVIIAAQHCRARLKN
PPKTASEYLETLERQGLAKSCNGNS

Nucleotide

• Length: 558 bp

>T180441 NZ_CP085345:424605-424985 [Cupriavidus sp. MP-37]
ATGAGCGTGCTCGTCGACACTTCGGTGTGGGTGAGCCATTTCCGGCACCGCAACGACGCCCTGATCCGCCTGATGGAGGC
GGACCAGGTGCTGATCCACCCCATGGTCGTGGCGGAACTGGCCTGCGGCACGCCACCGGCGCCCCGGGCACAGACGCTCG
GCGACCTGGGGCTGTTGCAGGCGGCCAGGCAGGCGACGCAGGCAGAAGTCATCGCACTGGTCGAGCGCGAGCAACTGTAT
GGGCTGGGCTGCGGCCTGGTGGATGTCACGTTGCTAGCTTCGACCCTGATTACCCCCGGCGCCCGGCTTTGGACACTGGA
CCAGCGCCTGAACGCTTTGTCCGCCCGCTTTGGCGTGGGATATCGTCCTCGTCTCCACTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16618.01 Da
• Isoelectric Point: 5.9324

>AT180441 WP_183906422.1 NZ_CP064187:4057905-4058351 [Rhizobium sp. 007]
MSQLLERPILPSADDTELAAEASRYISRAKHEGAELRVQVDGGELLKLPKAVSELLYHLLTEMAQGNAVTLIPVHAELTT
QEAADHLNVSRPYLIKLLEEGKIPFHMVGSHRRIKFSDLDGFRRKAEDERKRLMDELASQAQDLGMGY

Nucleotide

• Length: 447 bp

>AT180441 NZ_CP085345:424402-424608 [Cupriavidus sp. MP-37]
ATGCGCACGACTGTCACGATCGATGACGCCCTGTATGAACAAGCCCTGGAGATGGCGGACCCCGGCATGGACAAAGCAGA
CCTGTTTCGCGAGGCAATCAAGACATTCGTTCGGGTGCAGGCCGCCAAGCGCCTCGCGGCCCTCGGCGGCGCCGCCCCCG
ACATGCAGGACATCCCCCGCCGCAGTCCCGAACCCGACGTGAAATGA