Detailed information of TA system TA180440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 715910..716484
• Replicon: chromosome
• Accession: NZ_CP064187
• Organism: Rhizobium sp. 007

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ISN39_RS03435
• Protein ID: WP_194729180.1
• Coordinates: 716119..716484 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: ISN39_RS03430
• Protein ID: WP_194729179.1
• Coordinates: 715910..716125 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISN39_RS03405	712123..713076	-	954	WP_194729176.1	sulfate adenylyltransferase subunit CysD	-
ISN39_RS03410	713235..713975	-	741	WP_194729177.1	phosphoadenylyl-sulfate reductase	-
ISN39_RS03415	714398..714844	+	447	WP_022716377.1	Rrf2 family transcriptional regulator	-
ISN39_RS03420	714984..715370	+	387	WP_028739821.1	hypothetical protein	-
ISN39_RS03425	715375..715851	+	477	WP_194729178.1	DUF2214 domain-containing protein	-
ISN39_RS03430	715910..716125	+	216	WP_194729179.1	ribbon-helix-helix domain-containing protein	Antitoxin
ISN39_RS03435	716119..716484	+	366	WP_194729180.1	type II toxin-antitoxin system VapC family toxin	Toxin
ISN39_RS03440	716922..718571	-	1650	WP_074066969.1	choline dehydrogenase	-
ISN39_RS03445	718633..720096	-	1464	WP_194729181.1	betaine-aldehyde dehydrogenase	-
ISN39_RS03450	720161..720745	-	585	WP_074066971.1	transcriptional regulator BetI	-
ISN39_RS03455	720888..721442	+	555	WP_194729182.1	HdeD family acid-resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13621.59 Da
• Isoelectric Point: 5.6194

>T180440 WP_194729180.1 NZ_CP064187:716119-716484 [Rhizobium sp. 007]
MVKALFDTNILIDYLNAIPEARDELDRYGRRAISVVTWMEVLIGADPDVEAATRSFLNNFQIIAVDNAIAEGAVRLRQHH
RINLPDAIIWSTADAHALLLVTRNTKDFPADNPGIRVPYLR

Nucleotide

• Length: 366 bp

>T180440 NZ_CP085345:c377759-377286 [Cupriavidus sp. MP-37]
TTGAAGCCGGCCTGGCGCATCGCCACCGACACGCCCGCCTACACCGCGGACGATGCCAGCGGTGCCGGCGCCAGGGCCAC
CGGAGGGCGCTGGAACCGGCAGGGAACGCCGCTGATCTATGCGGCGTCCAGCATCGCGCTGGCTTGCCTGGAAACGCTGG
TTCACCTGGGCTCGGCCGGGCTGCCGCTGAACCGCTACCTGGTTCGAATCGATATCCCTGATGACCTGTGGGCCGCGGCA
CGGCATCTGACGGCGGCCAGCGCGCCGGTCGGCTGGGATGCCATTCCCGCCGGCAAGACCAGCCTCGACACCGGCGAGGA
CTGGGTCCGCGAGGGCACCAGCGCCTTGCTGCTGGTGCCTTCCATCGTGGTTCCCGAAGAGTTCAACGTGCTAGTCAATC
CCTTGCACGCGGATGCCCGGCGGCTGGTCTATACCAAGGTTCGCCGCTGGCAGTACGACGCCCGCCTGGCCTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8306.51 Da
• Isoelectric Point: 8.6003

>AT180440 WP_194729179.1 NZ_CP064187:715910-716125 [Rhizobium sp. 007]
MRILVDIVESELKALDKMAKSEKVSRASLIRKAVNDFLDRHDRTKEAEAFGLWGNRKIDGLEYQDKVRSEW

Nucleotide

• Length: 216 bp

>AT180440 NZ_CP085345:c378271-377756 [Cupriavidus sp. MP-37]
ATGCCGCGCATGCTCACGCCCAAAGGCCATTATCCACTCGCAGCCGAACGCCGGCCCGCTCGCGAGCTCAGCCTCAATAG
CTATCTGGCGGTCTATGAGGCCACGCTGTCGACCGAAGGGGCCATGTCGGTGATCCACGCCATCCGTGAGGGCGTGCCGG
CTTCCGACCTCAACCGGCTGGCCGAATCCATGGGGACGTCCAAGGAGCAGCTGATCAAGACGCTGGACTTGCCGCGTGCC
ACCGTTGACCGCAAGGCGCGCGCGCACCAGAACCTGTCGAGCGAGCAGAGCGAGCGCGTGCTGGGCATGGTGCGGCTGGT
GGGCCAGGTGCAGGCGCTGGTGGAAAGCTCGGGCGATCCGCGCGGCTTCAATGCGCCACAGTGGGTGGCGCAGTGGCTGG
AGCAGCCGTCGCCGGCGCTGGGTGGGCAACGTCCCGCCGAGCTGATGGATACCGTCGCGGGCCAGCGCATCGTCTCCGAC
CTGGTGGCCAGGATGCAGAGCGGGGCCTACGCTTGA