Detailed information of TA system TA180439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4705665..4706293
• Replicon: chromosome
• Accession: NZ_CP064180
• Organism: Citrobacter amalonaticus strain CA71

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A7L6Z8W6
• Locus tag: ISX58_RS22785
• Protein ID: WP_044257173.1
• Coordinates: 4705665..4706051 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: ISX58_RS22790
• Protein ID: WP_161215612.1
• Coordinates: 4706051..4706293 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISX58_RS22765	4701018..4702355	+	1338	WP_194694161.1	VWA domain-containing protein	-
ISX58_RS22770	4702373..4703869	+	1497	WP_044257176.1	HAMP domain-containing histidine kinase	-
ISX58_RS22775	4703874..4704560	+	687	WP_044257174.1	response regulator transcription factor	-
ISX58_RS22780	4704705..4705634	+	930	WP_042998866.1	formate dehydrogenase accessory protein FdhE	-
ISX58_RS22785	4705665..4706051	-	387	WP_044257173.1	type II toxin-antitoxin system VapC family toxin	Toxin
ISX58_RS22790	4706051..4706293	-	243	WP_161215612.1	ribbon-helix-helix domain-containing protein	Antitoxin
ISX58_RS22795	4706501..4707427	+	927	WP_044257169.1	alpha/beta hydrolase	-
ISX58_RS22800	4707442..4708383	-	942	WP_042998862.1	fatty acid biosynthesis protein FabY	-
ISX58_RS22805	4708428..4708865	-	438	WP_044257167.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ISX58_RS22810	4708862..4709734	-	873	WP_044257165.1	virulence factor BrkB family protein	-
ISX58_RS22815	4709728..4710327	-	600	WP_044257163.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14403.70 Da
• Isoelectric Point: 9.4114

>T180439 WP_044257173.1 NZ_CP064180:c4706051-4705665 [Citrobacter amalonaticus]
MQKGPVLFDTNILIDLFSGRQEAQQVLEAYPPQNAISLITWMEVMVGAKKYHQEYRTRVALSAFNIIGVSLEIAERSVNL
RQEYGMKLPDAIILATAQIHRFALVTRNTRDFAGIPGVITPYQLQAGR

Nucleotide

• Length: 387 bp

>T180439 NZ_CP085344:2564109-2564486 [Cupriavidus sp. MP-37]
ATGCCAGCCAAAGAAAGACCCCTCTTTTGGATCGGAAGAAGCAGAAAGGACCTGACGGAGCTTCCGCTCGACGTGCGCAG
GTTTTTCGGCCATGCGCTGCATTTTGCCCAGCGCGGTGATCGACACGATGCGGCGAAGATTCTCAAGGGTTTTGGCAGCG
CCGGGGTGCTCGAAATCCTGGAGGACGACGCTGCCGGCACCTACCGCGCGGTCTATACCGTGAAATTCGCTGAAGCGGTG
TTCGTCCTTCACTGCTTCCAGAAGAAGAGCAAACGCGGCATTGCCACGCCGAAGGCAGACATCGAAATCATTCGCGCCAG
GCTCAAGGTCGCAGAAGCACTGGCATGGGAGTTACGACATGGCAAAACGCAGGATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9335.53 Da
• Isoelectric Point: 4.2075

>AT180439 WP_161215612.1 NZ_CP064180:c4706293-4706051 [Citrobacter amalonaticus]
IMAGMDMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISNALGLWQGCEEDGVEYQRTLREEW

Nucleotide

• Length: 243 bp

>AT180439 NZ_CP085344:2564467-2564802 [Cupriavidus sp. MP-37]
ATGGCAAAACGCAGGATTGATGGCATCGAGGTCGAGGCCAGCTCCGGCAACGTGTTTGCCGACCTGGGCGTGCCGGAGGC
CGACAAGCTGAAAATCAAGTCCGGACTGGCCATCGAGATCACGCGCGCCGTACGCAGCCTTGGCCTGACCCAGGAAGAAG
CAGCCCACCGCATGGGCATCCCGCAACCGAAGGTCTCCGGACTGCTTCGCGGCGACTTCGCCAACCTGTCGGAGCGCAAG
CTGATGGACTGCCTGAACCGGCTTGGATACGACATCGAGATCAAGGTCAGCCCGGCGCTGGAACCGATCGGCCACCTCAC
GCTGAAGGTCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7L6Z8W6