Detailed information of TA system TA180427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 1852578..1853397
• Replicon: chromosome
• Accession: NZ_CP064180
• Organism: Citrobacter amalonaticus strain CA71

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: A0A3S5G4N2
• Locus tag: ISX58_RS08650
• Protein ID: WP_043001077.1
• Coordinates: 1852578..1852835 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: ISX58_RS08655
• Protein ID: WP_044258114.1
• Coordinates: 1852846..1853397 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISX58_RS08625	1848384..1849289	-	906	WP_194694231.1	dihydrodipicolinate synthase family protein	-
ISX58_RS08630	1849294..1850082	-	789	WP_043001081.1	IclR family transcriptional regulator	-
ISX58_RS08635	1850318..1851109	+	792	WP_044258121.1	PhzF family phenazine biosynthesis protein	-
ISX58_RS08640	1851590..1851982	+	393	WP_044258118.1	VOC family protein	-
ISX58_RS08645	1851992..1852198	+	207	WP_044258115.1	tryptophan synthase subunit alpha	-
ISX58_RS08650	1852578..1852835	+	258	WP_043001077.1	YjhX family toxin	Toxin
ISX58_RS08655	1852846..1853397	+	552	WP_044258114.1	N-acetyltransferase	Antitoxin
ISX58_RS08660	1853449..1854195	+	747	WP_044258113.1	class I SAM-dependent methyltransferase	-
ISX58_RS08665	1854872..1855456	+	585	WP_044258110.1	hypothetical protein	-
ISX58_RS08670	1855767..1856891	-	1125	WP_043001073.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9490.00 Da
• Isoelectric Point: 11.1381

>T180427 WP_043001077.1 NZ_CP064180:1852578-1852835 [Citrobacter amalonaticus]
MNLSRQEQRTLHVLAKGGRIAHVRDASGRVTAVECYSREGLLLADCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QPDNR

Nucleotide

• Length: 258 bp

>T180427 NZ_CP085294:971669-971785 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20365.25 Da
• Isoelectric Point: 6.0668

>AT180427 WP_044258114.1 NZ_CP064180:1852846-1853397 [Citrobacter amalonaticus]
MTTRNFTIHITDESDTNDIREVETRAFGYSKEACLVASLLEDDTARPTLSLLARQEGNAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGRGVGGLLIRTGIEHLRSMGCQCVFVLGHATYYPRHGFEPCAGDKGFPAPYPIAEEHKACWMMQSLSA
QPFDRAGDIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT180427 NZ_CP085294:c971901-971696 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAGTAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Structures

Toxin

• AlphaFold DB: A0A3S5G4N2