180426

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1256318..1256938 Replicon chromosome
Accession NZ_CP064180
Organism Citrobacter amalonaticus strain CA71

Toxin (Protein)


Gene name Hha Uniprot ID V1H8E6
Locus tag ISX58_RS05930 Protein ID WP_001280991.1
Coordinates 1256318..1256536 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3Q8DH98
Locus tag ISX58_RS05935 Protein ID WP_042324455.1
Coordinates 1256564..1256938 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISX58_RS05900 1251971..1252567 + 597 WP_044258943.1 membrane protein -
ISX58_RS05905 1252650..1253003 + 354 WP_043001561.1 DUF1428 family protein -
ISX58_RS05910 1253043..1254593 - 1551 WP_181825318.1 EAL domain-containing protein -
ISX58_RS05915 1254816..1254956 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
ISX58_RS05920 1255006..1255476 - 471 WP_043001559.1 YlaC family protein -
ISX58_RS05925 1255590..1256141 - 552 WP_044258934.1 maltose O-acetyltransferase -
ISX58_RS05930 1256318..1256536 - 219 WP_001280991.1 hemolysin expression modulator Hha Toxin
ISX58_RS05935 1256564..1256938 - 375 WP_042324455.1 Hha toxicity modulator TomB Antitoxin
ISX58_RS05940 1257449..1260598 - 3150 WP_043001557.1 multidrug efflux RND transporter permease subunit -
ISX58_RS05945 1260621..1261814 - 1194 WP_043001556.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8613.97 Da        Isoelectric Point: 8.9008

>T180426 WP_001280991.1 NZ_CP064180:c1256536-1256318 [Citrobacter amalonaticus]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T180426 NZ_CP085294:c802280-802185 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14495.31 Da        Isoelectric Point: 4.8989

>AT180426 WP_042324455.1 NZ_CP064180:c1256938-1256564 [Citrobacter amalonaticus]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT180426 NZ_CP085294:802086-802150 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATCAGTAGTAGGGTGTCTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4ICG

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q8DH98

References