Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 11386..12029 Replicon plasmid p19110124-4
Accession NZ_CP064176
Organism Klebsiella pneumoniae strain 19110124

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag ISU39_RS27070 Protein ID WP_000754566.1
Coordinates 11386..11802 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag ISU39_RS27075 Protein ID WP_001261276.1
Coordinates 11799..12029 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU39_RS27045 6582..8081 + 1500 Protein_8 Tn3 family transposase -
ISU39_RS27050 8244..8327 - 84 Protein_9 SOS response-associated peptidase -
ISU39_RS27055 8847..8948 + 102 Protein_10 Tn3 family transposase -
ISU39_RS27060 8968..10044 - 1077 WP_000227969.1 IS110 family transposase -
ISU39_RS27065 10326..11182 - 857 Protein_12 IS3-like element ISEc15 family transposase -
ISU39_RS27070 11386..11802 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
ISU39_RS27075 11799..12029 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ISU39_RS27080 12603..12953 + 351 WP_000493378.1 hypothetical protein -
ISU39_RS27085 13004..13747 + 744 WP_000129823.1 hypothetical protein -
ISU39_RS27090 13744..14520 + 777 WP_000015958.1 site-specific integrase -
ISU39_RS27095 14578..14835 - 258 WP_000764642.1 hypothetical protein -
ISU39_RS27100 14964..15068 - 105 WP_032409716.1 hypothetical protein -
ISU39_RS27105 15598..16464 + 867 WP_004118283.1 replication initiation protein -
ISU39_RS27110 16641..16910 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IVa / aph(4)-Ia - 1..22246 22246


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T180425 WP_000754566.1 NZ_CP064176:c11802-11386 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T180425 NZ_CP085294:361782-361925 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTAAAAGAAAACAATAAAAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT180425 WP_001261276.1 NZ_CP064176:c12029-11799 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT180425 NZ_CP085294:c362119-361857 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTACA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATATCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTTTTCTTTTACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References