Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53668..54404 Replicon plasmid p19110124-1
Accession NZ_CP064175
Organism Klebsiella pneumoniae strain 19110124

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag ISU39_RS26380 Protein ID WP_003026803.1
Coordinates 53922..54404 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag ISU39_RS26375 Protein ID WP_003026799.1
Coordinates 53668..53934 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU39_RS26330 49730..50092 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
ISU39_RS26335 50142..50492 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
ISU39_RS26340 50850..51119 + 270 WP_004152102.1 hypothetical protein -
ISU39_RS26345 51107..51682 + 576 WP_004152103.1 hypothetical protein -
ISU39_RS26350 51713..52207 + 495 WP_100774791.1 DNA-binding protein -
ISU39_RS26355 52251..52619 + 369 WP_004152105.1 hypothetical protein -
ISU39_RS26360 52653..52856 + 204 WP_004152106.1 hemolysin expression modulator Hha -
ISU39_RS26365 52905..53162 + 258 WP_004152107.1 hypothetical protein -
ISU39_RS26370 53238..53492 + 255 WP_004152108.1 hypothetical protein -
ISU39_RS26375 53668..53934 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
ISU39_RS26380 53922..54404 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
ISU39_RS26385 54605..56008 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
ISU39_RS26390 56037..56669 - 633 WP_001567369.1 hypothetical protein -
ISU39_RS26395 56883..57089 + 207 Protein_58 helix-turn-helix domain-containing protein -
ISU39_RS26400 57287..59050 + 1764 WP_004200842.1 DUF262 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / aph(3')-Ia - 1..168672 168672
- flank IS/Tn - - 54605..56008 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T180424 WP_003026803.1 NZ_CP064175:53922-54404 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T180424 NZ_CP085294:361782-361925 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTAAAAGAAAACAATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT180424 WP_003026799.1 NZ_CP064175:53668-53934 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT180424 NZ_CP085294:c361682-361484 [Enterococcus faecalis]
TTTTTAGGTAATTGTGCTATAATGGTAATGAAAAGAGAGCTATGCTGTAACATACCTCTCTGATGTAGAGCCGTTTAAGA
CGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATT
TCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References