Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5270734..5271359 Replicon chromosome
Accession NZ_CP064174
Organism Klebsiella pneumoniae strain 19110124

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag ISU39_RS25880 Protein ID WP_002882817.1
Coordinates 5270976..5271359 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ISU39_RS25875 Protein ID WP_004150355.1
Coordinates 5270734..5270976 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU39_RS25850 5266725..5267324 + 600 WP_004151865.1 glucose-1-phosphatase -
ISU39_RS25855 5267318..5268178 + 861 WP_002882807.1 virulence factor BrkB family protein -
ISU39_RS25860 5268175..5268612 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
ISU39_RS25865 5268657..5269598 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
ISU39_RS25870 5269612..5270529 - 918 WP_002882812.1 alpha/beta hydrolase -
ISU39_RS25875 5270734..5270976 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ISU39_RS25880 5270976..5271359 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
ISU39_RS25885 5271533..5272462 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ISU39_RS25890 5272459..5273094 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
ISU39_RS25895 5273091..5273993 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T180423 WP_002882817.1 NZ_CP064174:5270976-5271359 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T180423 NZ_CP085294:361411-361551 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT180423 WP_004150355.1 NZ_CP064174:5270734-5270976 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT180423 NZ_CP085294:c361682-361484 [Enterococcus faecalis]
TTTTTAGGTAATTGTGCTATAATGGTAATGAAAAGAGAGCTATGCTGTAACATACCTCTCTGATGTAGAGCCGTTTAAGA
CGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATT
TCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References