Detailed information of TA system TA180405

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1343498..1344071
• Replicon: chromosome
• Accession: NZ_CP064173
• Organism: Vibrio cyclitrophicus strain ED008

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q5DZS7
• Locus tag: ISX50_RS21205
• Protein ID: WP_011263487.1
• Coordinates: 1343498..1343812 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B6ERG3
• Locus tag: ISX50_RS21210
• Protein ID: WP_012551865.1
• Coordinates: 1343817..1344071 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISX50_RS21180 (ISX50_21150)	1338638..1339159	+	522	WP_248375164.1	gluconokinase	-
ISX50_RS21185 (ISX50_21155)	1339156..1340952	+	1797	WP_248398898.1	phosphogluconate dehydratase	-
ISX50_RS21190 (ISX50_21160)	1341026..1342033	-	1008	WP_248398900.1	LacI family DNA-binding transcriptional regulator	-
ISX50_RS21195 (ISX50_21165)	1342178..1342879	-	702	WP_248398902.1	lipoate--protein ligase	-
ISX50_RS21200 (ISX50_21170)	1343080..1343349	+	270	WP_248398904.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ISX50_RS21205 (ISX50_21175)	1343498..1343812	-	315	WP_011263487.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISX50_RS21210 (ISX50_21180)	1343817..1344071	-	255	WP_012551865.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ISX50_RS21215 (ISX50_21185)	1344154..1344245	-	92	Protein_1110	DUF3265 domain-containing protein	-
ISX50_RS21220 (ISX50_21190)	1344270..1344746	-	477	WP_248398906.1	GNAT family N-acetyltransferase	-
ISX50_RS21225 (ISX50_21195)	1344902..1345621	-	720	WP_248398908.1	hypothetical protein	-
ISX50_RS21235 (ISX50_21205)	1347100..1347438	+	339	WP_248398912.1	hypothetical protein	-
ISX50_RS21240 (ISX50_21210)	1347800..1348189	+	390	WP_248398914.1	phage integrase N-terminal SAM-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	1343080..1345621	2541

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-65)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12204.10 Da
• Isoelectric Point: 4.5467

>T180405 WP_011263487.1 NZ_CP064173:c1343812-1343498 [Vibrio cyclitrophicus]
MAVINWTESALLDLDEIAEYIAINNILAAKALVQSVFHRVERLEDFPLSGRSVPELPHLDYREIVLPPCRIFYKVTNDDI
VYILYVMREERELRQFVLNDRGLQ

Nucleotide

• Length: 315 bp

>T180405 NZ_CP085289:1562727-1563002 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9590.14 Da
• Isoelectric Point: 7.3180

>AT180405 WP_012551865.1 NZ_CP064173:c1344071-1343817 [Vibrio cyclitrophicus]
MKVELVTTLKRQATKILSELHASKEPVLITEHGQPSAYLLDVDDYEFMQKRIAILEGIARGEKAISDNRVVSHEVAKEKM
NKWL

Nucleotide

• Length: 255 bp

>AT180405 NZ_CP085289:1562468-1562734 [Enterococcus faecalis]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Structures

Toxin

• AlphaFold DB: Q5DZS7

Antitoxin

• AlphaFold DB: B6ERG3