180403

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 78355..78880 Replicon plasmid unnamed2
Accession NZ_CP064169
Organism Escherichia coli O157:H7 strain Wll001

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag IMX21_RS27605 Protein ID WP_001159868.1
Coordinates 78355..78660 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag IMX21_RS27610 Protein ID WP_000813634.1
Coordinates 78662..78880 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMX21_RS27585 74180..74518 - 339 WP_071525396.1 RepB family plasmid replication initiator protein -
IMX21_RS27590 74480..75693 + 1214 WP_164848152.1 IS3 family transposase -
IMX21_RS27595 75738..76825 + 1088 Protein_80 IS3 family transposase -
IMX21_RS27600 77548..78354 - 807 WP_000016989.1 site-specific integrase -
IMX21_RS27605 78355..78660 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
IMX21_RS27610 78662..78880 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IMX21_RS27615 79339..80022 + 684 WP_010891291.1 DNA-binding protein -
IMX21_RS27620 80019..80639 + 621 WP_001248529.1 hypothetical protein -
IMX21_RS27625 80636..80836 + 201 WP_000708307.1 hypothetical protein -
IMX21_RS27630 81244..82221 + 978 WP_000361615.1 RepB family plasmid replication initiator protein -
IMX21_RS27635 82506..83246 - 741 WP_001066920.1 tyrosine-type recombinase/integrase -
IMX21_RS27640 83367..83597 - 231 WP_010891288.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - exeG / exeE / stcE / espP / toxB / hlyD / hlyB / hlyA / hlyC 1..95164 95164


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T180403 WP_001159868.1 NZ_CP064169:c78660-78355 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T180403 NZ_CP085289:1056271-1056414 [Enterococcus faecalis]
ATGAATGTAAGTGCTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
CTCTTTTATCGCAACGTTAATCTTCGGAATCCTAAAAGCTGTTAAAGAAGACAAGAAAAAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT180403 WP_000813634.1 NZ_CP064169:c78880-78662 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT180403 NZ_CP085289:c1056576-1056346 [Enterococcus faecalis]
AGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTATAATAGCATTGAAAAGAGAGATATGCTGTAACAT
ACCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATTTATAAAAAATAACCGTACTCGCTAAAGTAGAC
GGTTATTTTTTCTTGTCTTCTTTAACAGCTTTTAGGATTCCGAAGATTAACGTTGCGATAAAAGAGCCGAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References