Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 31552..32111 | Replicon | plasmid unnamed2 |
| Accession | NZ_CP064169 | ||
| Organism | Escherichia coli O157:H7 strain Wll001 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O82928 |
| Locus tag | IMX21_RS27350 | Protein ID | WP_000421248.1 |
| Coordinates | 31552..31827 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | IMX21_RS27355 | Protein ID | WP_001178089.1 |
| Coordinates | 31827..32111 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IMX21_RS27315 | 26905..27294 | - | 390 | WP_001172748.1 | cytochrome b562 family protein | - |
| IMX21_RS27320 | 27338..29548 | - | 2211 | WP_000592771.1 | catalase/peroxidase KatP | - |
| IMX21_RS27325 | 29725..29910 | + | 186 | WP_001302179.1 | hypothetical protein | - |
| IMX21_RS27330 | 29910..30326 | + | 417 | Protein_27 | IS91 family transposase | - |
| IMX21_RS27335 | 30326..30892 | + | 567 | Protein_28 | IS3-like element IS600 family transposase | - |
| IMX21_RS27340 | 30936..31151 | + | 216 | Protein_29 | transposase | - |
| IMX21_RS27345 | 31251..31457 | - | 207 | WP_001105064.1 | hypothetical protein | - |
| IMX21_RS27350 | 31552..31827 | - | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| IMX21_RS27355 | 31827..32111 | - | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| IMX21_RS27360 | 33023..33880 | - | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| IMX21_RS27365 | 33873..33947 | - | 75 | WP_001370046.1 | RepA leader peptide Tap | - |
| IMX21_RS27370 | 34183..34437 | - | 255 | WP_000083831.1 | replication regulatory protein RepA | - |
| IMX21_RS27375 | 34677..35015 | - | 339 | WP_000766796.1 | hypothetical protein | - |
| IMX21_RS27380 | 35053..35262 | - | 210 | WP_001302200.1 | hemolysin expression modulator Hha | - |
| IMX21_RS27385 | 35308..35769 | - | 462 | WP_001233853.1 | thermonuclease family protein | - |
| IMX21_RS27390 | 36014..36226 | - | 213 | WP_001302189.1 | ANR family transcriptional regulator | - |
| IMX21_RS27395 | 36359..36919 | - | 561 | WP_000840472.1 | fertility inhibition protein FinO | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | exeG / exeE / stcE / espP / toxB / hlyD / hlyB / hlyA / hlyC | 1..95164 | 95164 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10735.42 Da Isoelectric Point: 9.9324
>T180402 WP_000421248.1 NZ_CP064169:c31827-31552 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T180402 NZ_CP085289:c1050841-1050500 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGCAGAGACGCCTTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGTCATAGAAACCCATGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGCAGAGACGCCTTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGTCATAGAAACCCATGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT180402 WP_001178089.1 NZ_CP064169:c32111-31827 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT180402 NZ_CP085289:c1051071-1050841 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAACTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA
ATGCTAACAACTAAATCAAGAAGACAAGGAAACTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6FAZ0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |