Detailed information of TA system TA180395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PsyrTA/-
• Location: 3477294..3479622
• Replicon: chromosome
• Accession: NZ_CP064167
• Organism: Escherichia coli O157:H7 strain Wll001

Toxin (Protein)

• Gene name: PsyrT
• Uniprot ID: A0A6N0MHC8
• Locus tag: IMX21_RS16895
• Protein ID: WP_000532146.1
• Coordinates: 3477294..3478268 (+)
• Length: 325 a.a.

Antitoxin (Protein)

• Gene name: PsyrA
• Uniprot ID: -
• Locus tag: IMX21_RS16900
• Protein ID: WP_001303704.1
• Coordinates: 3478327..3479622 (+)
• Length: 432 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMX21_RS16870	3473151..3474146	+	996	WP_000730263.1	gluconate operon transcriptional repressor GntR	-
IMX21_RS16875	3474285..3474812	+	528	WP_000108330.1	gluconokinase	-
IMX21_RS16880	3474816..3476156	+	1341	WP_000210111.1	gluconate transporter	-
IMX21_RS16885	3476296..3476904	+	609	WP_000410808.1	ATP-dependent DNA helicase	-
IMX21_RS16890	3476889..3477290	+	402	WP_000502498.1	ATP-dependent DNA helicase	-
IMX21_RS16895	3477294..3478268	+	975	WP_000532146.1	ATP-dependent DNA helicase	Toxin
IMX21_RS16900	3478327..3479622	+	1296	WP_001303704.1	DNA-protecting protein DprA	Antitoxin
IMX21_RS16905	3479668..3480261	-	594	WP_001002544.1	NAAT family transporter YhgN	-
IMX21_RS16910	3480453..3481556	+	1104	WP_000799956.1	aspartate-semialdehyde dehydrogenase	-
IMX21_RS16915	3481829..3484015	+	2187	WP_001283734.1	1,4-alpha-glucan branching enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• DNA recombination-mediator protein A (115-429)

Sequences

Toxin

Protein

• Length: 325 a.a.
• Molecular weight: 36460.93 Da
• Isoelectric Point: 9.2667

>T180395 WP_000532146.1 NZ_CP064167:3477294-3478268 [Escherichia coli O157:H7]
VGRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQRTNLRYGQIEKALKLLVAENP
SPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELADVESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDI
DSGLLHAANLFIKHADLSLNLNKQVAAGAFTQYGFKGNLPASLQGSTGRILSRWGYSGWGKQVAQEKKTGRFSDELVEAC
AEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFESTSTWFLILPGDWRRNLAYLLLMPLKKSWTIHRRKCSKTVSTSVKIS
TGRL

Nucleotide

• Length: 975 bp

>T180395 NZ_CP085282:756048-756794 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 432 a.a.
• Molecular weight: 47053.95 Da
• Isoelectric Point: 7.2590

>AT180395 WP_001303704.1 NZ_CP064167:3478327-3479622 [Escherichia coli O157:H7]
MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLLTSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAE
LLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLI
AGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENG
ASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESISIERLASCT
GLTIEQISVWLNRAEEEGRVIRLGEGHYQFR

Nucleotide

• Length: 1296 bp

>AT180395 NZ_CP085282:756795-756965 [Bacillus amyloliquefaciens]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: A0A6N0MHC8