Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3411051..3411682 Replicon chromosome
Accession NZ_CP064167
Organism Escherichia coli O157:H7 strain Wll001

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag IMX21_RS16525 Protein ID WP_001259386.1
Coordinates 3411051..3411326 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag IMX21_RS16530 Protein ID WP_000593555.1
Coordinates 3411323..3411682 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMX21_RS16510 3406055..3407122 + 1068 WP_000361458.1 HlyD family secretion protein -
IMX21_RS16515 3407119..3409854 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
IMX21_RS16520 3409854..3410978 + 1125 WP_001301659.1 ABC transporter permease -
IMX21_RS16525 3411051..3411326 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
IMX21_RS16530 3411323..3411682 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
IMX21_RS16535 3411732..3412592 - 861 WP_001131788.1 class II aldolase -
IMX21_RS16540 3412624..3412893 - 270 WP_000933060.1 HPr family phosphocarrier protein -
IMX21_RS16545 3412883..3414391 - 1509 WP_001301773.1 carbohydrate kinase -
IMX21_RS16550 3414384..3415742 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
IMX21_RS16555 3415819..3416100 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
IMX21_RS16560 3416097..3416570 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T180393 WP_001259386.1 NZ_CP064167:3411051-3411326 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T180393 NZ_CP085203:177520-177825 [Shigella flexneri]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT180393 WP_000593555.1 NZ_CP064167:3411323-3411682 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT180393 NZ_CP085203:177300-177518 [Shigella flexneri]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References