Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2264789..2265339 Replicon chromosome
Accession NZ_CP064167
Organism Escherichia coli O157:H7 strain Wll001

Toxin (Protein)


Gene name ccdB Uniprot ID D3H432
Locus tag IMX21_RS11350 Protein ID WP_001160975.1
Coordinates 2264789..2265103 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag IMX21_RS11355 Protein ID WP_000125566.1
Coordinates 2265106..2265339 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMX21_RS11325 2260464..2261750 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
IMX21_RS11330 2261750..2262739 + 990 WP_000241259.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
IMX21_RS11335 2262736..2263557 + 822 WP_001065380.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
IMX21_RS11340 2263560..2263937 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
IMX21_RS11345 2263944..2264792 + 849 WP_000257187.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
IMX21_RS11350 2264789..2265103 - 315 WP_001160975.1 CcdB family protein Toxin
IMX21_RS11355 2265106..2265339 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
IMX21_RS11360 2265425..2265904 - 480 WP_000624372.1 type 3 dihydrofolate reductase -
IMX21_RS11365 2266096..2267958 - 1863 WP_000377184.1 glutathione-regulated potassium-efflux system protein KefC -
IMX21_RS11370 2267951..2268481 - 531 WP_000600700.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
IMX21_RS11375 2268589..2269920 - 1332 WP_001183177.1 MFS transporter -
IMX21_RS11380 2269978..2270265 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.76 Da        Isoelectric Point: 9.8517

>T180388 WP_001160975.1 NZ_CP064167:c2265103-2264789 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T180388 NZ_CP085202:c4394270-4394163 [Shigella flexneri]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT180388 WP_000125566.1 NZ_CP064167:c2265339-2265106 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT180388 NZ_CP085202:4394327-4394382 [Shigella flexneri]
TCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References