Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 596353..596991 Replicon chromosome
Accession NZ_CP064167
Organism Escherichia coli O157:H7 strain Wll001

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag IMX21_RS03290 Protein ID WP_000813794.1
Coordinates 596815..596991 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag IMX21_RS03285 Protein ID WP_001270286.1
Coordinates 596353..596769 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMX21_RS03265 591505..592446 - 942 WP_001251320.1 ABC transporter permease -
IMX21_RS03270 592447..593460 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
IMX21_RS03275 593478..594623 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
IMX21_RS03280 594868..596274 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
IMX21_RS03285 596353..596769 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
IMX21_RS03290 596815..596991 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
IMX21_RS03295 597213..597443 + 231 WP_000494244.1 YncJ family protein -
IMX21_RS03300 597535..599496 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
IMX21_RS03305 599569..600105 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
IMX21_RS03310 600158..601369 + 1212 WP_071525382.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T180373 WP_000813794.1 NZ_CP064167:c596991-596815 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T180373 NZ_CP085200:705973-706419 [Mycobacterium ulcerans]
ATGGCGAAACTTTCCGGGTCGATCGACGTGCCGCTGTCTCCGGACGAGGCATGGATGCACGCATCCGATCTCTCCCGCTT
CAAGGAGTGGCTGACGATCCACCGAGTGTGGCGCAGCACGTTGCCCGAAACCCTCGAAAAGGGTGCGGTCGTCGAATCAA
TCGTGCAGGTCAAGGGCATGCACAACCGCATCAAATGGACCATCGTGCGCTACCAGCCGCCGGAAGGCATGACCCTCAAC
GGTGACGGGGTCGGCGGCGTCAAGGTCAAGCTGATGGCCAAGGTTGCAGCCGGAGAACAAGGCTCGATCGTCAGCTTCGA
CGTGCACCTTGGTGGGCCGGCTCTGTTCGGTCCGATCGGCATGGTCGTCGCCGCGGCGCTGCGCAGCGATATCAACGCAT
CGCTGCGGAACTTCGTCACCGTATTCGCCCCGCCGGCTGCCGGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT180373 WP_001270286.1 NZ_CP064167:c596769-596353 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT180373 NZ_CP085200:705780-705968 [Mycobacterium ulcerans]
ATGGGAATCCTGGACAAGGCGAAAGACCTGCTGGCGCAGAACGCCGACAAGGTCGAGACGGTGATCGACAAGGCCGGCGA
ATTCGTCGACGAGAAGACCGAAGGGAAGTACTCCGACACCATCCACAAGGTGCAAGAGGGGGCCAAGAAAGTCGCTGGTC
CCGGCGCCGCGGGCGAGCAGCAGAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References