Detailed information of TA system TA180368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 67377..68020
• Replicon: plasmid pM911-1.2
• Accession: NZ_CP064131
• Organism: Klebsiella pneumoniae strain M911-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: IOD52_RS26060
• Protein ID: WP_186474563.1
• Coordinates: 67377..67793 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: IOD52_RS26065
• Protein ID: WP_001261276.1
• Coordinates: 67790..68020 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD52_RS26045	62882..63901	+	1020	WP_186474561.1	type 1 fimbrial protein	-
IOD52_RS26050	64098..65621	-	1524	WP_117282697.1	EAL domain-containing protein	-
IOD52_RS26055	66056..67282	-	1227	Protein_61	IS3 family transposase	-
IOD52_RS26060	67377..67793	-	417	WP_186474563.1	type II toxin-antitoxin system VapC family toxin	Toxin
IOD52_RS26065	67790..68020	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IOD52_RS26070	68594..68944	+	351	WP_000493378.1	hypothetical protein	-
IOD52_RS26075	68995..69738	+	744	WP_000129823.1	hypothetical protein	-
IOD52_RS26080	69735..70511	+	777	WP_000015958.1	site-specific integrase	-
IOD52_RS26085	70569..70826	-	258	WP_000764642.1	hypothetical protein	-
IOD52_RS26090	70955..71059	-	105	WP_032409716.1	hypothetical protein	-
IOD52_RS26095	71594..72460	+	867	WP_194524182.1	replication initiation protein	-
IOD52_RS26100	72820..73014	-	195	Protein_70	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..85824	85824

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15078.58 Da
• Isoelectric Point: 8.5403

>T180368 WP_186474563.1 NZ_CP064131:c67793-67377 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T180368 NZ_CP085197:2558163-2558265 [Klebsiella quasipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT180368 WP_001261276.1 NZ_CP064131:c68020-67790 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT180368 NZ_CP085197:c2558269-2558122 [Klebsiella quasipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG

Structures

Antitoxin

• AlphaFold DB: A0A387K023