Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4431658..4432468 Replicon chromosome
Accession NZ_CP064129
Organism Klebsiella pneumoniae strain M911-1

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag IOD52_RS21660 Protein ID WP_004178461.1
Coordinates 4431935..4432468 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag IOD52_RS21655 Protein ID WP_002887278.1
Coordinates 4431658..4431924 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD52_RS21635 4427530..4427874 - 345 WP_021462623.1 cation efflux system protein CusF -
IOD52_RS21640 4427892..4429277 - 1386 WP_032417819.1 efflux transporter outer membrane subunit -
IOD52_RS21645 4429449..4430132 + 684 WP_004214143.1 copper response regulator transcription factor CusR -
IOD52_RS21650 4430122..4431555 + 1434 WP_023283180.1 Cu(+)/Ag(+) sensor histidine kinase -
IOD52_RS21655 4431658..4431924 + 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
IOD52_RS21660 4431935..4432468 + 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
IOD52_RS21665 4432516..4433637 - 1122 WP_004214138.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T180366 WP_004178461.1 NZ_CP064129:4431935-4432468 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T180366 NZ_CP085197:c97503-97222 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGCTTGGCGAGACGGTCAAAAAACAGTTCAAAAA
TAATCTCCAGCAGATTATGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCAAGC
TTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAAAGA
GAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT180366 WP_002887278.1 NZ_CP064129:4431658-4431924 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT180366 NZ_CP085197:c97735-97493 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGACTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGTCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAGCGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References