Detailed information of TA system TA180362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3563684..3564423
• Replicon: chromosome
• Accession: NZ_CP064129
• Organism: Klebsiella pneumoniae strain M911-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: IOD52_RS17545
• Protein ID: WP_021312536.1
• Coordinates: 3563684..3564169 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: IOD52_RS17550
• Protein ID: WP_003026799.1
• Coordinates: 3564157..3564423 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD52_RS17520	3558813..3560168	+	1356	WP_004901270.1	aromatic acid/H+ symport family MFS transporter	-
IOD52_RS17525	3560414..3560704	+	291	WP_002921931.1	HTH-type transcriptional regulator	-
IOD52_RS17530	3560956..3561168	+	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
IOD52_RS17535	3561267..3562886	-	1620	WP_004901278.1	ATP-binding cassette domain-containing protein	-
IOD52_RS17540	3563188..3563340	-	153	WP_002922102.1	type I toxin-antitoxin system toxin HokA	-
IOD52_RS17545	3563684..3564169	-	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
IOD52_RS17550	3564157..3564423	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
IOD52_RS17555	3564556..3564984	-	429	WP_004901287.1	GFA family protein	-
IOD52_RS17570	3567817..3569045	+	1229	WP_148116659.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T180362 WP_021312536.1 NZ_CP064129:c3564169-3563684 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T180362 NZ_CP085190:c71998-71693 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTCTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT180362 WP_003026799.1 NZ_CP064129:c3564423-3564157 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT180362 NZ_CP085190:c72218-72000 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATACAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66