Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /COG5642-COG5654
Location 2145574..2146540 Replicon chromosome
Accession NZ_CP064128
Organism Yersinia pestis strain M2086

Toxin (Protein)


Gene name - Uniprot ID A0A0E8XJV6
Locus tag ISU65_RS09505 Protein ID WP_002210981.1
Coordinates 2145574..2146032 (-) Length 153 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q66A69
Locus tag ISU65_RS09510 Protein ID WP_002210982.1
Coordinates 2146094..2146540 (-) Length 149 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU65_RS09475 2141388..2142053 + 666 WP_002210975.1 phage shock protein PspA -
ISU65_RS09480 2142215..2142442 + 228 WP_002210976.1 envelope stress response membrane protein PspB -
ISU65_RS09485 2142442..2142801 + 360 WP_002210977.1 envelope stress response membrane protein PspC -
ISU65_RS09490 2142886..2143128 + 243 WP_002216375.1 phage shock protein PspD -
ISU65_RS09495 2143109..2144506 + 1398 WP_002210979.1 YcjX family protein -
ISU65_RS09500 2144503..2145564 + 1062 WP_002210980.1 YcjF family protein -
ISU65_RS09505 2145574..2146032 - 459 WP_002210981.1 RES family NAD+ phosphorylase Toxin
ISU65_RS09510 2146094..2146540 - 447 WP_002210982.1 DUF2384 domain-containing protein Antitoxin
ISU65_RS09515 2146876..2148453 + 1578 WP_002210983.1 transcriptional regulator TyrR -
ISU65_RS09520 2148600..2149058 - 459 WP_002213775.1 IS200/IS605-like element IS1541B family transposase -
ISU65_RS09525 2149311..2149814 - 504 WP_002210984.1 thiol peroxidase -
ISU65_RS09530 2149977..2150951 + 975 WP_002210985.1 L-Ala-D/L-Glu epimerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2148600..2149058 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(2-147)

Antitoxin

Antitoxin Xre

(15-147)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 16994.38 Da        Isoelectric Point: 4.8732

>T180338 WP_002210981.1 NZ_CP064128:c2146032-2145574 [Yersinia pestis]
MTLYRIVMRRYLASAWTGYGAEIYGGRWNHKGHAAIYLASSISLAMLETLAHIQDSSTLSEFELFQIEISDSSIMLLQPQ
DWPTDWRSDPAPVATMDVGTEWLESESSVGLLVPSTLVPSENNLLVNPHHKDFPACLASVKPLSFVFDPRLK

Download         Length: 459 bp

>T180338 NZ_CP085186:48714-48863 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 149 a.a.        Molecular weight: 16401.94 Da        Isoelectric Point: 9.7155

>AT180338 WP_002210982.1 NZ_CP064128:c2146540-2146094 [Yersinia pestis]
MRAYRPNPATKMGTLWREIGLPASRGTILVDSIKMGFSVDVIDSIHLWASMPKAEILRATGIPSRSLTRRRTHDGRFTPE
ESERIARFVRVMDAAVDLFGGDKGKAITWMSTPIKGLGYRSPDSLLETETGALEVCDLIGRLEHGVFT

Download         Length: 447 bp

>AT180338 NZ_CP085186:c48669-48610 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E8XJV6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8XFL1

References