Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3395266..3395886 Replicon chromosome
Accession NZ_CP064122
Organism Yersinia pestis strain M-1770

Toxin (Protein)


Gene name Hha Uniprot ID Q66DR5
Locus tag ISU59_RS15050 Protein ID WP_002208622.1
Coordinates 3395683..3395886 (+) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q66DR4
Locus tag ISU59_RS15045 Protein ID WP_002218472.1
Coordinates 3395266..3395634 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU59_RS15030 3393461..3393721 + 261 WP_002208617.1 type B 50S ribosomal protein L31 -
ISU59_RS15035 3393737..3393880 + 144 WP_002208618.1 type B 50S ribosomal protein L36 -
ISU59_RS15040 3394754..3395107 + 354 WP_002208619.1 hypothetical protein -
ISU59_RS15045 3395266..3395634 + 369 WP_002218472.1 Hha toxicity modulator TomB Antitoxin
ISU59_RS15050 3395683..3395886 + 204 WP_002208622.1 expression modulating protein YmoA Toxin
ISU59_RS15060 3396774..3397163 + 390 WP_002208623.1 MGMT family protein -
ISU59_RS15065 3397299..3397820 - 522 WP_002208624.1 lipoprotein -
ISU59_RS15070 3398077..3398937 + 861 WP_002208625.1 acyl-CoA thioesterase II -
ISU59_RS15075 3399197..3400348 - 1152 Protein_2955 ammonium transporter AmtB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8063.31 Da        Isoelectric Point: 6.4573

>T180271 WP_002208622.1 NZ_CP064122:3395683-3395886 [Yersinia pestis]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Download         Length: 204 bp

>T180271 NZ_CP085095:c1325847-1325665 [Aggregatibacter actinomycetemcomitans]
ATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAA
GGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCA
AAAAAGACTTAAACTTAAAATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14215.00 Da        Isoelectric Point: 4.6121

>AT180271 WP_002218472.1 NZ_CP064122:3395266-3395634 [Yersinia pestis]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWINDPTSAINLQLNDLIEHIASFVMSFKIKYPDGSQLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKEHLFRLFSGEYVCTLMKT

Download         Length: 369 bp

>AT180271 NZ_CP085095:c1325613-1325194 [Aggregatibacter actinomycetemcomitans]
ATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGT
AAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATG
ATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAA
GTCTTCTTACTCAATGAAATGCTCGCACAAGGTGTGCGAAAAGCTGAAATGGCGCGCCGTCTTGATGTTCACATGCCGCA
AGTCGATCGCTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAA
TTAATTTATCGGTTCATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2K5S

Antitoxin

Source ID Structure
AlphaFold DB Q66DR4

References